Yang Chin-An, Liang Chao, Lin Chia-Li, Hsiao Chiung-Tzu, Peng Ching-Tien, Lin Hung-Chih, Chang Jan-Gowth
Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
Division of General Pediatrics, Children's Hospital of China Medical University, Taichung, Taiwan.
PLoS Negl Trop Dis. 2017 Sep 25;11(9):e0005963. doi: 10.1371/journal.pntd.0005963. eCollection 2017 Sep.
Previous studies on the association of enterobiasis and chronic inflammatory diseases have revealed contradictory results. The interaction of Enterobius vermicularis infection in particular with gut microbiota and induced immune responses has never been thoroughly examined.
METHODOLOGY/FINDINGS: In order to answer the question of whether exposure to pinworm and mebendazole can shift the intestinal microbial composition and immune responses, we recruited 109 (30 pinworm-negative, 79 pinworm-infected) first and fourth grade primary school children in Taichung, Taiwan, for a gut microbiome study and an intestinal cytokine and SIgA analysis. In the pinworm-infected individuals, fecal samples were collected again at 2 weeks after administration of 100 mg mebendazole. Gut microbiota diversity increased after Enterobius infection, and it peaked after administration of mebendazole. At the phylum level, pinworm infection and mebendazole deworming were associated with a decreased relative abundance of Fusobacteria and an increased proportion of Actinobacteria. At the genus level, the relative abundance of the probiotic Bifidobacterium increased after enterobiasis and mebendazole treatment. The intestinal SIgA level was found to be lower in the pinworm-infected group, and was elevated in half of the mebendazole-treated group. A higher proportion of pre-treatment Salmonella spp. was associated with a non-increase in SIgA after mebendazole deworming treatment.
CONCLUSIONS/SIGNIFICANCE: Childhood exposure to pinworm plus mebendazole is associated with increased bacterial diversity, an increased abundance of Actinobacteria including the probiotic Bifidobacterium, and a decreased proportion of Fusobacteria. The gut SIgA level was lower in the pinworm-infected group, and was increased in half of the individuals after mebendazole deworming treatment.
先前关于蛲虫病与慢性炎症性疾病关联的研究结果相互矛盾。尤其是蛲虫感染与肠道微生物群及诱导的免疫反应之间的相互作用从未得到彻底研究。
方法/发现：为了回答接触蛲虫和甲苯咪唑是否会改变肠道微生物组成及免疫反应这一问题，我们招募了台湾台中市109名（30名蛲虫阴性、79名蛲虫感染）一、四年级小学生进行肠道微生物组研究以及肠道细胞因子和分泌型免疫球蛋白A（SIgA）分析。在蛲虫感染个体中，服用100毫克甲苯咪唑2周后再次采集粪便样本。蛲虫感染后肠道微生物群多样性增加，并在服用甲苯咪唑后达到峰值。在门水平上，蛲虫感染和甲苯咪唑驱虫与梭杆菌门相对丰度降低及放线菌门比例增加有关。在属水平上，益生菌双歧杆菌属的相对丰度在蛲虫病和甲苯咪唑治疗后增加。发现蛲虫感染组的肠道SIgA水平较低，而在一半的甲苯咪唑治疗组中有所升高。治疗前较高比例的沙门氏菌属与甲苯咪唑驱虫治疗后SIgA未增加有关。
结论/意义：儿童时期接触蛲虫加甲苯咪唑与细菌多样性增加、包括益生菌双歧杆菌在内的放线菌丰度增加以及梭杆菌比例降低有关。蛲虫感染组的肠道SIgA水平较低，而在一半个体中甲苯咪唑驱虫治疗后有所升高。
