Chen Yumin, Fritz Ronald D, Kock Lindsay, Garg Dinesh, Davis R Mark, Kasturi Prabhakar
PepsiCo, Inc. Global R&D Measurement Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
PepsiCo, Inc. Global R&D Measurement Sciences, 617 W. Main Street, Barrington, IL 60010, USA.
Food Chem. 2018 Feb 1;240:391-395. doi: 10.1016/j.foodchem.2017.07.153. Epub 2017 Jul 29.
A step-wise, 'test-all-positive-gluten' analytical methodology has been developed and verified to assess kernel-based gluten contamination (i.e., wheat, barley and rye kernels) during gluten-free (GF) oat production. It targets GF claim compliance at the serving-size level (of a pouch or approximately 40-50g). Oat groats are collected from GF oat production following a robust attribute-based sampling plan then split into 75-g subsamples, and ground. R-Biopharm R5 sandwich ELISA R7001 is used for analysis of all the first15-g portions of the ground sample. A >20-ppm result disqualifies the production lot, while a >5 to <20-ppm result triggers complete analysis of the remaining 60-g of ground sample, analyzed in 15-g portions. If all five 15-g test results are <20ppm, and their average is <10.67ppm (since a 20-ppm contaminant in 40g of oats would dilute to 10.67ppm in 75-g), the lot is passed.
一种逐步的“检测所有阳性麸质”分析方法已被开发并验证，用于评估无麸质（GF）燕麦生产过程中基于谷粒的麸质污染（即小麦、大麦和黑麦谷粒）。它针对每份食用量（一袋或约40 - 50克）的无麸质声明合规性。按照严格的基于属性的抽样计划，从无麸质燕麦生产中收集燕麦粒，然后分成75克的子样本并研磨。R-Biopharm R5夹心ELISA R7001用于分析研磨后样本的所有前15克部分。结果大于20 ppm会使该生产批次不合格，而结果大于5至小于20 ppm会触发对剩余60克研磨样本进行完整分析，以15克部分进行分析。如果所有五个15克的测试结果均小于20 ppm，且它们的平均值小于10.67 ppm（因为40克燕麦中20 ppm的污染物在75克中会稀释至10.67 ppm），则该批次通过。
