Zhang Ye, Zhao Chengying, Tian Guifang, Lu Chang, Li Yuzhi, He Lili, Xiao Hang, Zheng Jinkai
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Department of Food Science, University of Massachusetts, Amherst, MA 01003, United States.
Food Chem. 2018 Feb 1;240:743-750. doi: 10.1016/j.foodchem.2017.07.103. Epub 2017 Jul 25.
Rapid detection of bioactive components in food has attracted great attention. Herein, we report a novel method through surface-enhanced Raman spectroscopy (SERS) spectra based barcodes for simultaneous characterization of chemical structures and bioactivities of nine citrus components for the first time. SERS barcodes were successfully used to characterize and discriminate all the components with high sensitivity down to 40-60ng. Importantly, SERS barcodes exhibited the 'identity' characteristics. Beyond the molecular structure information, bioactivity information can also be read from the barcodes according to the bioactivity assay and structure-activity relationship. Hence, a simple and intuitive SERS barcoding approach used for simultaneous characterization of chemical structures and bioactivities was established. With a large database of barcodes, there is high potential that the SERS barcoding approach could be further developed to be a rapid, simple and effective foodomics-like approach for bioactive compound identification from a complex food matrix.
食品中生物活性成分的快速检测备受关注。在此，我们首次报道了一种基于表面增强拉曼光谱（SERS）光谱条形码的新方法，用于同时表征九种柑橘成分的化学结构和生物活性。SERS条形码已成功用于以高达40 - 60纳克的高灵敏度对所有成分进行表征和鉴别。重要的是，SERS条形码展现出“身份”特征。除了分子结构信息外，还可根据生物活性测定和构效关系从条形码中读取生物活性信息。因此，建立了一种用于同时表征化学结构和生物活性的简单直观的SERS条形码方法。凭借大量的条形码数据库，SERS条形码方法具有很大潜力，可进一步发展成为一种从复杂食品基质中鉴定生物活性化合物的快速、简单且有效的类似食品组学的方法。
