Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Laboratory of Chemistry of Natural Molecules, Gembloux Agro-Bio Tech, University of Liege, Gembloux 5030, Belgium.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Food Chem. 2021 Feb 15;338:128115. doi: 10.1016/j.foodchem.2020.128115. Epub 2020 Sep 17.
Citrus flavonoids consist of diverse analogs and possess various health-promoting effects dramatically depending on their chemical structures. Since different flavonoids usually co-exist in real samples, it's necessary to develop rapid and efficient methods for simultaneous determination of multiple flavonoids. Thin layer chromatography combined with surface enhanced Raman spectroscopy (TLC-SERS) was established to simultaneously separate and detect 14 citrus flavonoids for the first time. These target compounds could be characterized and discriminated when paired with SERS at 6-500 times greater the sensitivity than TLC alone. TLC-SERS exhibited high recovery rates (91.5-121.7%) with relative standard deviation lower than 20.8%. Moreover, the established TLC-SERS method was successfully used to simultaneously detect multiple flavonoids in real samples, which exhibited comparable accuracy to high performance liquid chromatography with shorter analytical time (10 vs 45 min). All the results demonstrated that this could be a promising method for simultaneous, rapid, sensitive and accurate detection of flavonoids.
柑橘类黄酮由多种类似物组成，根据其化学结构具有不同的促进健康的作用。由于不同的类黄酮通常在实际样品中共存，因此有必要开发快速有效的方法来同时测定多种类黄酮。首次建立了薄层色谱结合表面增强拉曼光谱（TLC-SERS）同时分离和检测 14 种柑橘类黄酮的方法。当与 SERS 结合时，这些目标化合物可以被表征和区分，其灵敏度比单独的 TLC 高 6-500 倍。TLC-SERS 表现出较高的回收率（91.5-121.7%），相对标准偏差低于 20.8%。此外，所建立的 TLC-SERS 方法成功地用于同时检测实际样品中的多种类黄酮，其分析时间（10 分钟对 45 分钟）更短，准确性与高效液相色谱相当。所有结果表明，这可能是一种用于同时、快速、灵敏和准确检测类黄酮的有前途的方法。
