Fu Jining, Cui Yongyuan
Surgical Department, Siping City Centre People's Hospital, Siping, Jilin - PR China.
Department of Clinical Laboratory, Beijing CapitalBio lndependent Clinical Laboratory, Beijing - PR China.
Int J Biol Markers. 2017 Oct 31;32(4):e428-e433. doi: 10.5301/ijbm.5000303.
ZEB1-AS1 acts as an oncogene in hepatocellular carcinoma, accelerating tumor growth and promoting metastasis. However, its roles in colorectal cancer (CRC) remain unclear.
In this study, we determined the expression of ZEB1-AS1 in CRC tissues by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, we investigated the relationship between various clinicopathological features of CRC patients and ZEB1-AS1 expression, and evaluated the diagnostic and prognostic value of ZEB1-AS1 in CRC.
We found that ZEB1-AS1 expression was significantly higher in CRC tissues than in adjacent normal colorectal tissues. Moreover, its expression was significantly correlated with tumor size, differentiation degree, TNM grade, metastasis, depth of invasion and Dukes' classification, but not with sex, age, location and organization. In addition, at the optimal cutoff value of 2.340, the values of diagnostic sensitivity and specificity amounted to 63.0% and 90.7%, respectively, with an area under the curve (AUC) of 0.846 (95% CI, 0.797-0.895). Finally, CRC patients of the high ZEB1-AS1 expression group had a poorer prognosis and a significantly lower survival rate than those of the low expression group, and Cox regression analysis indicated that ZEB1-AS1 expression and metastasis were independent predictors of poor prognosis.
Our data suggest that ZEB1-AS1 has no obvious early diagnostic value, but it may be utilized as a new prognostic biomarker for CRC.
ZEB1-AS1在肝细胞癌中作为一种癌基因发挥作用，加速肿瘤生长并促进转移。然而，其在结直肠癌（CRC）中的作用仍不清楚。
在本研究中，我们通过定量实时聚合酶链反应（qRT-PCR）测定了CRC组织中ZEB1-AS1的表达。此外，我们研究了CRC患者各种临床病理特征与ZEB1-AS1表达之间的关系，并评估了ZEB1-AS1在CRC中的诊断和预后价值。
我们发现CRC组织中ZEB1-AS1的表达明显高于相邻正常结直肠组织。此外，其表达与肿瘤大小、分化程度、TNM分级、转移、浸润深度和Dukes分期显著相关，但与性别、年龄、位置和组织类型无关。此外，在最佳临界值2.340时，诊断敏感性和特异性分别为63.0%和90.7%，曲线下面积（AUC）为0.846（95%CI，0.797-0.895）。最后，ZEB1-AS1高表达组的CRC患者预后较差，生存率明显低于低表达组，Cox回归分析表明ZEB1-AS1表达和转移是预后不良的独立预测因素。
我们的数据表明，ZEB1-AS1没有明显的早期诊断价值，但它可能作为CRC的一种新的预后生物标志物。
