Department of Chemistry, ‡Environmental Toxicology Program, and §Department of Biochemistry and Molecular Biology, University of California-Riverside , Riverside, California 92521, United States.
Anal Chem. 2017 Oct 17;89(20):11113-11121. doi: 10.1021/acs.analchem.7b03377. Epub 2017 Oct 5.
A simple three component array of host-fluorophore complexes is capable of sensitive and selective discrimination of heavy metal ions, including lanthanide and actinide salts in aqueous solution. Instead of applying optical sensors that only use "single-mode" detection, i.e., coordination of the metal to a specific ligand and monitoring the change in emission of an appended fluorophore, we exploit a series of host-fluorophore complexes that are affected by the presence of small amounts of metal ions in aqueous solution in different ways. Variable host-metal and host-guest-metal interactions lead to both turn-on and turn-off fluorescence sensing mechanisms, enhancing the discriminatory properties of the array. The limit of detection for certain metals is as low as 70 nM, and highly similar metals such as lanthanides and actinides can be easily distinguished at low micromolar concentrations in complex salt mixtures.
一个简单的三组分主体-荧光团配合物阵列能够灵敏和选择性地识别重金属离子，包括水溶液中的镧系和锕系盐。我们没有采用仅使用“单模”检测的光学传感器，即金属与特定配体的配位以及监测附加荧光团的发射变化，而是利用一系列主体-荧光团配合物，这些配合物受水溶液中少量金属离子的存在以不同的方式影响。可变的主体-金属和主体-客体-金属相互作用导致开-关荧光传感机制，增强了阵列的区分性质。某些金属的检测限低至 70 nM，并且在复杂盐混合物中，即使在低微摩尔浓度下，也可以轻松区分高度相似的金属，如镧系和锕系元素。
