Aissa S, Chabchoub N, Abdelmalek R, Kanoun F, Goubantini A, Ammari L, Kilani B, Bouratbine A, Tiouiri-Ben Aissa H, Aoun K
Service des maladies infectieuses, Hôpital la Rabta, Jabbari-Jebel Lakhdar, 1007, Tunis, Tunisie, Laboratoire de recherche LR 11-IPT-06 « Parasitologie médicale, biotechnologie et biomolecules », Institut pasteur de Tunis, Université Tunis El Manar, Faculté de médecine de Tunis, Tunisie.
Laboratoire de recherche LR 11-IPT-06 « Parasitologie médicale, biotechnologie et biomolecules », Institut pasteur de Tunis.
Med Sante Trop. 2017 Aug 1;27(3):281-285. doi: 10.1684/mst.2017.0697.
Asymptomatic carriage of microsporidia (ACM) has not been described in patients living with HIV (PLHIV) in Tunisia. To determine the prevalence of ACM in PLHIV followed at Tunis la Rabta hospital, describe its clinical features and course, and identify the species involved. This prospective study (2005-2009) included 71 asymptomatic PLHIV compared with 37 PLHIV with diarrhea. One stool sample per patient was examined by microscopy after Weber staining and by PCR. Species identification was confirmed by specific PCR and sequencing. In cases of ACM, a second stool sample was examined in 2010 and a clinical check-up took place in 2013. The prevalence of ACM in asymptomatic PLHIV was 11.3 % (8/71). PCR was more sensitive than microscopy (P = 0.0047). ACM was associated with stage C of HIV infection (P = 0.008) and CD4 T cells <100/μl (P = 0.033). The species involved were E. intestinalis (6 cases) and E. bieneusi (2 cases). Six PLHIV remained asymptomatic with negative stool examinations, but two developed digestive signs. ACM is common among Tunisian PLHIV and it appears to be associated with E. intestinalis.
突尼斯的艾滋病病毒感染者（PLHIV）中尚未有微孢子虫无症状携带（ACM）的相关描述。为了确定在突尼斯拉巴塔医院接受治疗的PLHIV中ACM的患病率，描述其临床特征和病程，并识别其中涉及的物种。这项前瞻性研究（2005 - 2009年）纳入了71名无症状PLHIV，并与37名腹泻的PLHIV进行比较。每位患者的一份粪便样本经韦伯染色后通过显微镜检查，并进行聚合酶链反应（PCR）检测。通过特异性PCR和测序确认物种鉴定结果。对于ACM病例，2010年检查了第二份粪便样本，并于2013年进行了临床检查。无症状PLHIV中ACM的患病率为11.3%（8/71）。PCR比显微镜检查更敏感（P = 0.0047）。ACM与HIV感染的C期（P = 0.008）和CD4 T细胞<100/μl（P = 0.033）相关。涉及的物种为肠脑炎微孢子虫（6例）和比氏肠微孢子虫（2例）。6名PLHIV粪便检查呈阴性且无症状，但有2名出现了消化系统症状。ACM在突尼斯的PLHIV中很常见，且似乎与肠脑炎微孢子虫有关。
