雄性大鼠肝脏中特异性表达的细胞色素P-450（M-1）的基因结构

Gene structure of cytochrome P-450(M-1) specifically expressed in male rat liver.

作者信息

Morishima N, Yoshioka H, Higashi Y, Sogawa K, Fujii-Kuriyama Y

机构信息

Department of Biochemistry, Japanese Foundation for Cancer Research, Tokyo.

出版信息

Biochemistry. 1987 Dec 15;26(25):8279-85. doi: 10.1021/bi00399a039.

DOI:10.1021/bi00399a039

PMID:2894840

Abstract

Cytochrome P-450(M-1) [P-450(M-1)] is specifically expressed in adult male rat liver [Yoshioka, H., Morohashi, K., Sogawa, K., Miyata, T., Kawajiri, K., Hirose, T., Inayama, S., Fujii-Kuriyama, Y., & Omura, T. (1987) J. Biol. Chem. 262, 1706-1711]. Isolation and analysis of the gene for P-450(M-1) revealed that the coding region of the gene is interrupted by eight introns and is dispersed over a 35-kilobase pair region of chromosomal DNA. Intron insertion sites of the P-450(M-1) gene are located at equivalent positions to those of cytochrome P-450b and P-450e, which are phenobarbital-inducible. Sequence analysis of the 5'-upstream region of the P-450(M-1) gene shows that there is a homologous sequence to glucocorticoid regulatory elements (GRE) identified in glucocorticoid-responsive genes.

摘要

细胞色素P-450（M-1）[P-450（M-1）]在成年雄性大鼠肝脏中特异性表达[吉冈，H.，诸桥，K.，曾川，K.，宫田，T.，川尻，K.，广濑，T.，稻山，S.，藤井-栗山，Y.，& 大村，T.（1987年）《生物化学杂志》262卷，1706 - 1711页]。对P-450（M-1）基因的分离和分析表明，该基因的编码区被8个内含子打断，分布在染色体DNA的35千碱基对区域内。P-450（M-1）基因的内含子插入位点与苯巴比妥诱导的细胞色素P-450b和P-450e的内含子插入位点位于相同位置。对P-450（M-1）基因5'上游区域的序列分析表明，存在与糖皮质激素反应基因中鉴定出的糖皮质激素调节元件（GRE）的同源序列。