Rangarajan P N, Ravishankar H, Padmanaban G
Biochem Biophys Res Commun. 1987 Apr 14;144(1):258-63. doi: 10.1016/s0006-291x(87)80504-1.
A cytochrome P-450e gene variant has been isolated from the rat liver genomic library. It is a typical e gene clone but unique in having b-like single base substitutions at specific sites in the 5' flanking region. It also appears to have certain additional restriction sites in the introns. When compared with the cytochrome P-450b gene, the e gene has some of the repetitive motifs interrupted in the 5' flanking region. In addition, this region is characterized by the presence of alternating pyrimidine-purine stretch, steroid hormone regulatory elements, consensus eukaryotic enhancer sequence and sequences involved in general amino acid regulation.
一种细胞色素P - 450e基因变体已从大鼠肝脏基因组文库中分离出来。它是一个典型的e基因克隆，但独特之处在于其5'侧翼区域的特定位点存在b样单碱基取代。它在内含子中似乎也有某些额外的限制性酶切位点。与细胞色素P - 450b基因相比，e基因在5'侧翼区域的一些重复基序被打断。此外，该区域的特征是存在交替的嘧啶 - 嘌呤序列、类固醇激素调节元件、共有真核增强子序列以及参与一般氨基酸调节的序列。
