Kamiya Yukiko, Yamada Yoshinobu, Muro Takahiro, Matsuura Kazunori, Asanuma Hiroyuki
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
Institute of Materials and Systems for Sustainability, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
ChemMedChem. 2017 Dec 19;12(24):2016-2021. doi: 10.1002/cmdc.201700512. Epub 2017 Oct 18.
In this study we constructed spherical photo-responsive microcapsules composed of three photo-switchable DNA strands. These strands first formed a three-way junction (TWJ) motif that further self-assembled to form microspheres through hybridization of the sticky-end regions of each branch. To serve as the photo-switch, multiple unmodified azobenzene (Azo) or 2,6-dimethyl-4-(methylthio)azobenzene (SDM-Azo) were introduced into the sticky-end regions via a d-threoninol linker. The DNA capsule structure deformed upon trans-to-cis isomerization of Azo or SDM-Azo induced by specific light irradiation. In addition, photo-triggered release of encapsulated small molecules from the DNA microcapsule was successfully achieved. Moreover, we demonstrated that photo-triggered release of doxorubicin caused cytotoxicity to cultured cells. This biocompatible photo-responsive microcapsule has potential application as a photo-controlled drug-release system.
在本研究中，我们构建了由三条光开关DNA链组成的球形光响应微胶囊。这些链首先形成一个三向连接（TWJ）基序，该基序通过每个分支粘性末端区域的杂交进一步自组装形成微球。为了用作光开关，通过d-苏糖醇连接子将多个未修饰的偶氮苯（Azo）或2,6-二甲基-4-（甲硫基）偶氮苯（SDM-Azo）引入粘性末端区域。在特定光照射诱导的Azo或SDM-Azo从反式到顺式异构化时，DNA胶囊结构发生变形。此外，成功实现了从DNA微胶囊中光触发释放封装的小分子。此外，我们证明了光触发释放阿霉素对培养细胞具有细胞毒性。这种生物相容性光响应微胶囊作为光控药物释放系统具有潜在的应用价值。
