Polymer Chemistry Laboratory, Department of Applied Chemistry, Indian Institute of Technology (ISM) , Dhanbad 826004, India.
Biomaterials and Tissue Engineering Laboratory, School of Medical Science and Technology, Indian Institute of Technology , Kharagpur 721302, India.
ACS Appl Mater Interfaces. 2017 Oct 25;9(42):36583-36595. doi: 10.1021/acsami.7b10716. Epub 2017 Oct 12.
Recently, a novel biopolymeric nanocomposite hydrogel comprised of in situ formed silver nanowires (AgNWs) deposited chemically cross-linked carboxymethyl cellulose (CMC) has been developed, which demonstrates superior efficacy as anticancer drug-curcumin carrier. The cross-linked polymer has been prepared by grafting poly [2-(methacryloyloxy) ethyl trimethylammonium chloride] on CMC using diethylene glycol dimethacrylate cross-linker. The nanocomposite hydrogel has the capability to encapsulate both hydrophobic/hydrophilic transdermal drugs. With variation in reaction conditions/parameters, several composite materials have been synthesized and depending on lower swelling/higher cross-linking and greater gel strength, an optimized grade of nanocomposite hydrogel has been selected. The developed nanocomposite hydrogel is characterized with FTIR/NMR spectra, FESEM/XRD/TGA/AFM/XPS analyses, and UV-visible spectroscopy. Rheological study has been performed to enlighten the gel strength of the composite material. The synthesized nanocomposite hydrogel is biodegradable and nontoxic to mesenchymal stem cells (hMSCs). In vitro release of curcumin suggests that in situ incorporation of AgNWs on cross-linked CMC enhanced the penetration power of nanocomposite hydrogel and released the drug in sustained way (∼62% for curcumin released in 4 days). Ex vivo rat skin permeation study confirms that the drug from both the cross-linked and nanocomposite hydrogel was permeable through the rat skin in controlled fashion. Additionally the curcumin loaded composite hydrogel can efficiently kill the MG 63 cancer cells, which has been confirmed by apoptosis study and therefore, probably be a suitable carrier for curcumin delivery toward cancer cells.
最近，开发了一种新型的生物聚合物纳米复合水凝胶，由原位形成的银纳米线（AgNWs）化学交联羧甲基纤维素（CMC）组成，作为抗癌药物姜黄素载体具有优异的效果。交联聚合物是通过使用二甘醇二甲基丙烯酸酯交联剂将聚[2-（甲基丙烯酰氧基）乙基三甲基氯化铵]接枝到 CMC 上制备的。纳米复合水凝胶具有包封疏水性/亲水性透皮药物的能力。通过改变反应条件/参数，已经合成了几种复合材料，并根据较低的溶胀/较高的交联和更大的凝胶强度，选择了优化等级的纳米复合水凝胶。所开发的纳米复合水凝胶的特征在于 FTIR/NMR 光谱、FESEM/XRD/TGA/AFM/XPS 分析和 UV-可见光谱。进行了流变学研究以阐明复合材料的凝胶强度。所合成的纳米复合水凝胶是可生物降解的，对间充质干细胞（hMSCs）无毒。姜黄素的体外释放表明，原位掺入交联 CMC 上的 AgNWs 增强了纳米复合水凝胶的渗透能力，并以持续的方式释放药物（在 4 天内释放约 62%的姜黄素）。大鼠皮肤渗透研究证实，交联和纳米复合水凝胶中的药物都以受控的方式通过大鼠皮肤渗透。此外，负载有姜黄素的复合水凝胶可以有效地杀死 MG 63 癌细胞，这已通过细胞凋亡研究得到证实，因此，可能是姜黄素递送至癌细胞的合适载体。
