Department of Chemistry, Emory University , Atlanta, Georgia 30322, United States.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University , Atlanta, Georgia 30322, United States.
J Am Chem Soc. 2017 Oct 11;139(40):14025-14028. doi: 10.1021/jacs.7b08087. Epub 2017 Oct 2.
We describe the co-assembly of two different building units: collagen-mimetic peptides and DNA origami. Two peptides CP and sCP are designed with a sequence comprising a central block (Pro-Hyp-Gly) and two positively charged domains (Pro-Arg-Gly) at both N- and C-termini. Co-assembly of peptides and DNA origami two-layer (TL) nanosheets affords the formation of one-dimensional nanowires with repeating periodicity of ∼10 nm. Structural analyses suggest a face-to-face stacking of DNA nanosheets with peptides aligned perpendicularly to the sheet surfaces. We demonstrate the potential of selective peptide-DNA association between face-to-face and edge-to-edge packing by tailoring the size of DNA nanostructures. This study presents an attractive strategy to create hybrid biomolecular assemblies from peptide- and DNA-based building blocks that takes advantage of the intrinsic chemical and physical properties of the respective components to encode structural and, potentially, functional complexity within readily accessible biomimetic materials.
胶原模拟肽和 DNA 折纸。两种肽 CP 和 sCP 设计的序列包含一个中央块（Pro-Hyp-Gly）和两个正电荷域（Pro-Arg-Gly），分别位于 N-和 C-末端。肽和 DNA 折纸双层（TL）纳米片的共组装提供了具有约 10nm 重复周期性的一维纳米线的形成。结构分析表明 DNA 纳米片的面对面堆叠与垂直于片表面的肽排列。通过调整 DNA 纳米结构的大小，我们证明了在面对面和边缘对边缘包装之间进行选择性肽-DNA 缔合的潜力。本研究提出了一种有吸引力的策略，从基于肽和 DNA 的构建块创建混合生物分子组装，利用各自组件的固有化学和物理性质在易于获得的仿生材料中编码结构和潜在功能复杂性。
