College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China.
Bioresour Technol. 2023 Nov;387:129612. doi: 10.1016/j.biortech.2023.129612. Epub 2023 Aug 3.
Two mature anaerobic ammonium oxidation (anammox) consortia with high/low relative abundance of anammox bacteria were inoculated for the rapid sludge proliferation and biofilm formation in this study, named up-flow anaerobic sludge blanket reactor (UASB1) (high) and UASB2 (low), respectively. Results showed that the nitrogen removal efficiency of UASB2 reached 90.94% after the 120-day operation, which was 13% higher than that of UASB1. Moreover, its biomass amounts were 22.18% (biofilm) and 40.96% (flocs) higher than that of UASB1, respectively. Ca. Kuenenia possessed relative abundances of 29.32% (flocs), 27.42% (biofilm) and 31.56% (flocs), 35.20% (biofilm) in the UASB1 and UASB2, respectively. The relative abundances of genes involved in anammox transformation (hzs, nir) and carbon metabolism (fdh, lgA/B/C, acs) were higher in the UASB2, indicating that Ca. Kuenenia might produce acetate and glycogen to enhance microbial interactions. These findings emphasized the importance of microbial interactions in anammox sludge proliferation and biofilm formation.
本研究分别接种了具有高/低氨单加氧菌相对丰度的成熟厌氧氨氧化（anammox）生物群落，用于快速污泥增殖和生物膜形成，分别命名为上流式厌氧污泥床反应器（UASB1）（高）和 UASB2（低）。结果表明，UASB2 在 120 天的运行后，氮去除效率达到 90.94%，比 UASB1 高 13%。此外，其生物量分别比 UASB1 高出 22.18%（生物膜）和 40.96%（絮体）。Ca. Kuenenia 在 UASB1 和 UASB2 中的相对丰度分别为 29.32%（絮体）、27.42%（生物膜）和 31.56%（絮体）、35.20%（生物膜）。UASB2 中参与厌氧氨氧化转化（hzs、nir）和碳代谢（fdh、lgA/B/C、acs）的基因相对丰度较高，表明 Ca. Kuenenia 可能产生乙酸盐和糖原来增强微生物相互作用。这些发现强调了微生物相互作用在厌氧氨氧化污泥增殖和生物膜形成中的重要性。
