抗白细胞介素 33 抗体对 2,4-二硝基氯苯诱导的特应性皮炎小鼠模型有治疗作用。
Anti-IL-33 Antibody Has a Therapeutic Effect in an Atopic Dermatitis Murine Model Induced by 2, 4-Dinitrochlorobenzene.
机构信息
Department of Dermatology, Shengjing Hospital of China Medical University, No. 36 Sanhao St., Heping Area, Shenyang, Liaoning, 110004, China.
出版信息
Inflammation. 2018 Feb;41(1):154-163. doi: 10.1007/s10753-017-0673-7.
IL-33 is a new member of the IL-1 family that plays a role in allergic disease. In this study, we evaluated the potential on the inhibition of atopic dermatitis (AD) of anti-mouse IL-33 antibody (αIL-33Ab) using 2, 4-dinitrochlorobenzene (DNCB)-induced AD mice model. We treated mice with αIL-33Ab via subcutaneous injection of each DNCB treatment 1 h later from day 1 to day 33 for 14 times. A control group received tacrolimus. Skin lesion and scratching behavior were compared. Ear thickness, dermatitis score, eosinophils and mast cells infiltration, and serum IgE levels were also analyzed. Correlations between serum IL-33 as well as soluble(s) ST2 and AD disease activity index in human AD were also investigated. DNCB-induced AD-like mice treated with αIL-33Ab showed improved AD-like symptoms. Eosinophils and mast cells infiltration and serum IgE levels were also significantly reduced by αIL-33Ab. Our study suggests that blockade of IL-33 has a curative effect on AD.
白细胞介素-33（IL-33）是白细胞介素-1 家族的新成员，在过敏疾病中发挥作用。在这项研究中，我们评估了抗小鼠白细胞介素-33 抗体（αIL-33Ab）抑制特应性皮炎（AD）的潜力，使用 2,4-二硝基氯苯（DNCB）诱导的 AD 小鼠模型。我们在第 1 天至第 33 天的每 1 次 DNCB 处理后 1 小时通过皮下注射 αIL-33Ab 对小鼠进行治疗，共进行 14 次。对照组接受他克莫司。比较皮肤损伤和搔抓行为。还分析了耳厚度、皮炎评分、嗜酸性粒细胞和肥大细胞浸润以及血清 IgE 水平。还研究了人 AD 中血清 IL-33 以及可溶性（s）ST2 与 AD 疾病活动指数之间的相关性。用 αIL-33Ab 治疗的 DNCB 诱导的 AD 样小鼠显示出改善的 AD 样症状。αIL-33Ab 还显著降低了嗜酸性粒细胞和肥大细胞浸润以及血清 IgE 水平。我们的研究表明，阻断白细胞介素-33 对 AD 具有治疗作用。
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