利用游离型载体生成人诱导多能干细胞系CERAi001-A-6
Generation of a human induced pluripotent stem cell line CERAi001-A-6 using episomal vectors.
作者信息
Wong Raymond C B, Hung Sandy S, Jackson Stacey, Singh Vikrant, Khan Shahnaz, Liang Helena H, Kearns Lisa S, Nguyen Tu, Conquest Alison, Daniszewski Maciej, Hewitt Alex W, Pébay Alice
机构信息
Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia.
Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Australia; Ophthalmology, Department of Surgery, The University of Melbourne, Australia.
出版信息
Stem Cell Res. 2017 Jul;22:13-15. doi: 10.1016/j.scr.2017.05.007. Epub 2017 May 19.
We report the generation of the hiPSC line CERAi001-A-6 from primary human dermal fibroblasts. Reprogramming was performed using episomal vector delivery of OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA for p53.
我们报告了从原代人皮肤成纤维细胞中生成人诱导多能干细胞系CERAi001-A-6的过程。使用携带OCT4、SOX2、KLF4、L-MYC、LIN28的附加型载体以及针对p53的短发夹RNA进行重编程。
Zotero 插件