Pillalamarri Nirmala, Shalom Dara F, Pilkinton Marjorie L, Winkler Harvey A, Chatterjee Prodyot K, Solanki Malvika, Metz Christine N
Center for Immunology and Inflammation, The Feinstein Institute for Medical Research, Manhasset, NY.
Female Pelvic Med Reconstr Surg. 2018 Nov/Dec;24(6):449-453. doi: 10.1097/SPV.0000000000000492.
The aims of this study were to analyze levels of selected inflammatory urinary cytokines/chemokines in subjects with overactive bladder (OAB) and to determine if cytokine/chemokine levels correlate with quality of life and symptom distress.
This prospective, case-control pilot analysis included 23 women with OAB and 22 control subjects. Overactive bladder subjects were enrolled if they had symptoms of urinary frequency, urgency, or urge incontinence for more than 3 months and urodynamic evidence of detrusor overactivity. Control subjects denied urinary symptoms. Subjects and control subjects were excluded if they had known inflammatory bladder or systemic conditions, cystitis, stones, or recent anticholinergic use. Urine samples were collected from each subject and control. Subjects filled out the Incontinence Quality of Life Questionnaire and the Urinary Distress Inventory Questionnaire 6. Cytokine/chemokine levels were determined using the multiplexed Meso Scale Discovery Platform and were corrected for urinary creatinine concentrations. Statistical analysis comparing cytokine/chemokine levels was performed using the Mann-Whitney U test; relationships between cytokine/chemokine and questionnaire scores were calculated with Spearman correlation coefficient.
Subjects with OAB had significantly lower urinary interleukin 10 (IL-10), IL-12-p70, and IL-13 levels compared with control subjects. Interleukin 1 correlated with worsening symptom distress on Urinary Distress Inventory Questionnaire 6.
To our knowledge, this is at present the only study correlating inflammatory cytokine/chemokine levels in women with OAB with quality of life and distress. Interleukin 1 signified worsening distress, whereas IL-10, IL-12p70, and IL-13 were the only cytokines found at different levels in subjects. Our findings support a larger study in order to evaluate the value of urinary cytokines/chemokines as potential biomarkers.
本研究旨在分析膀胱过度活动症（OAB）患者尿液中特定炎性细胞因子/趋化因子的水平，并确定细胞因子/趋化因子水平是否与生活质量和症状困扰相关。
这项前瞻性病例对照初步分析纳入了23名OAB女性患者和22名对照者。膀胱过度活动症患者若有尿频、尿急或急迫性尿失禁症状超过3个月且有逼尿肌过度活动的尿动力学证据，则纳入研究。对照者否认有泌尿系统症状。若受试者和对照者已知患有炎性膀胱或全身性疾病、膀胱炎、结石或近期使用过抗胆碱能药物，则将其排除。从每位受试者和对照者收集尿液样本。受试者填写尿失禁生活质量问卷和泌尿困扰量表问卷6。使用多重微尺度发现平台测定细胞因子/趋化因子水平，并根据尿肌酐浓度进行校正。使用曼-惠特尼U检验对细胞因子/趋化因子水平进行统计学分析；用斯皮尔曼相关系数计算细胞因子/趋化因子与问卷得分之间的关系。
与对照者相比，OAB患者尿液中的白细胞介素10（IL-10）、IL-12-p70和IL-13水平显著降低。白细胞介素1与泌尿困扰量表问卷6中症状困扰加重相关。
据我们所知，这是目前唯一一项将OAB女性患者的炎性细胞因子/趋化因子水平与生活质量和困扰相关联的研究。白细胞介素1表明困扰加重，而IL-10、IL-12p70和IL-13是在受试者中发现的仅有的不同水平的细胞因子。我们的研究结果支持开展更大规模的研究，以评估尿液细胞因子/趋化因子作为潜在生物标志物的价值。
