Canadian Light Source, 44 Innovation Blvd, Saskatoon, SK S7N 2V3, Canada.
Currently Department of Crop Protection, Phytopathology Unit, Ecole Nationale d'Agriculture de Meknès, BP/S 40, Meknès 50001, Morocco.
Int J Mol Sci. 2017 Sep 26;18(10):2058. doi: 10.3390/ijms18102058.
Clubroot disease is a serious threat to canola production in western Canada and many parts of the world. is a clubroot resistance (CR) gene identified recently and its molecular mechanisms in mediating CR have been studied using several omics approaches. The current study aimed to characterize the biochemical changes in the cell wall of canola roots connecting to key molecular mechanisms of this CR gene identified in prior studies using Fourier transform infrared (FTIR) spectroscopy. The expression of nine genes involved in phenylpropanoid metabolism was also studied using qPCR. Between susceptible (S) and resistance (R) samples, the most notable biochemical changes were related to an increased biosynthesis of lignin and phenolics. These results were supported by the transcription data on higher expression of . The up-regulation of PAL is indicative of an inducible defence response conferred by ; the activation of this basal defence gene via the phenylpropanoid pathway may contribute to clubroot resistance conferred by . The data indicate that several cell-wall components, including lignin and pectin, may play a role in defence responses against clubroot. Principal components analysis of FTIR data separated non-inoculated samples from inoculated samples, but not so much between inoculated S and inoculated R samples. It is also shown that FTIR spectroscopy can be a useful tool in studying plant-pathogen interaction at cellular levels.
根肿病是加拿大西部和世界许多地区油菜生产的严重威胁。 是最近鉴定的一种根肿病抗性（CR）基因，其在介导 CR 中的分子机制已通过多种组学方法进行了研究。本研究旨在使用傅里叶变换红外（FTIR）光谱法，对与先前研究中鉴定的该 CR 基因的关键分子机制相关的油菜根与细胞壁的生化变化进行特征描述。还使用 qPCR 研究了 9 个与苯丙烷代谢相关的基因的表达。在感病（S）和抗病（R）样本之间，最显著的生化变化与木质素和酚类生物合成的增加有关。转录数据表明 基因的表达更高，支持了这些结果。PAL 的上调表明 赋予了可诱导的防御反应；通过苯丙烷途径激活该基础防御基因可能有助于赋予对根肿病的抗性。数据表明，包括木质素和果胶在内的几种细胞壁成分可能在防御根肿病的反应中发挥作用。FTIR 数据的主成分分析将未接种的样本与接种的样本分开，但未将接种的 S 样本和接种的 R 样本分开。还表明，FTIR 光谱学可以成为在细胞水平研究植物-病原体相互作用的有用工具。
