Zhang X, Li T, Niu X X, Wang S X, Liu Z H
Laboratory of Electron Microscopy, Peking University First Hospital, Beijing 100034, China.
Zhonghua Fu Chan Ke Za Zhi. 2017 Sep 25;52(9):618-622. doi: 10.3760/cma.j.issn.0529-567X.2017.09.008.
To investigate interactions of vaginal epithelial cells (VEC) with Candida albicans and the protective effect of Lactobacillus crispatus on VEC. VK2/E6E7 VEC cell line was cultured. Candida albicans and Lactobacillus crispatus suspension were prepared respectively. Ultrastructural morphological changes of VEC infected with Candida albicans and treated with Lactobacillus crispatus were observed by scanning electron microscope (SEM). Candida albicans invaded into VEC mainly through endocytosis after an early infection of 6 hours. Pseudopods of VEC increased, contacted and covered the surface of pseudohyphae. After 12 hours infection, active penetration developed into the predominant invasion mechanism. Candida albicans directly penetrated through VEC, destroyed the surface structures, and even led to cell death. A reduction of pseudopods on VEC was observed. After co-incubation with Lactobacillus crispatus, SEM revealed a morphological recovery of VEC and destruction of pseudohyphae. s Candida albicans invade VEC mainly through endocytosis and active penetration. Lactobacillus crispatus could utilize occupying effect to exert protective effect on VEC during the infection with Candida albicans.
研究阴道上皮细胞（VEC）与白色念珠菌的相互作用以及卷曲乳杆菌对VEC的保护作用。培养VK2/E6E7 VEC细胞系。分别制备白色念珠菌和卷曲乳杆菌悬液。通过扫描电子显微镜（SEM）观察感染白色念珠菌并经卷曲乳杆菌处理的VEC的超微结构形态变化。白色念珠菌在早期感染6小时后主要通过内吞作用侵入VEC。VEC的伪足增多，接触并覆盖假菌丝表面。感染12小时后，主动穿透发展成为主要的侵入机制。白色念珠菌直接穿透VEC，破坏表面结构，甚至导致细胞死亡。观察到VEC上的伪足减少。与卷曲乳杆菌共孵育后，SEM显示VEC形态恢复且假菌丝被破坏。白色念珠菌主要通过内吞作用和主动穿透侵入VEC。在白色念珠菌感染期间，卷曲乳杆菌可利用占位效应发挥对VEC的保护作用。
