Musik Irena, Kiełczykowska Małgorzata, Rajtar Barbara, Świątek Łukasz, Polz-Dacewicz Małgorzata, Kocot Joanna
Chair and Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
Department of Virology, Medical University of Lublin, Lublin, Poland.
Ann Agric Environ Med. 2017 Sep 21;24(3):423-427. doi: 10.26444/aaem/74473. Epub 2017 Jun 7.
Lithium is used in medicine but its application may cause diverse side effects. Selenium has been found to show protective properties against negative influence of different harmful factors. This study was aimed at evaluating the influence of non-toxic dose of lithium on antioxidant parameters in FaDu (ATCC HTB-43) and Vero (ECACC No. 84113001) cell lines as well as the possible protective effect of non-toxic concentration of sodium selenite.
The cells were subjected to 0.17 mmol/L of LiCO and/or 2.9 µmol/L of NaSeO · 5HO for Vero as well as 0.47 mmol/L of LiCO and/or 3.0 µmol/L of NaSeO · 5HO for FaDu cells. The incubation was continued for the subsequent 72 h. In the cells total antioxidant status (TAS) values, activities of antioxidant enzymes - superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as the reduced glutathione concentration (GSH) were determined.
In Vero cells lithium decreased all studied parameters, particularly GPx. Selenium co-treatment showed a distinct protective effect. In FaDu cells the similar effect was observed only in case of GSH. The results point to differences in action of lithium and selenium in physiological and pathological state. As long-term lithium therapy is applied in psychiatric patients the results regarding Vero line let suggest that selenium might be considered as an adjuvant alleviating side effects of Li-treatment.
锂在医学中被使用，但其应用可能会引起多种副作用。已发现硒对不同有害因素的负面影响具有保护特性。本研究旨在评估无毒剂量的锂对FaDu（ATCC HTB - 43）和Vero（ECACC No. 84113001）细胞系抗氧化参数的影响，以及亚硒酸钠无毒浓度可能的保护作用。
将Vero细胞分别用0.17 mmol/L的LiCO₃和/或2.9 µmol/L的Na₂SeO₃·5H₂O处理，将FaDu细胞用0.47 mmol/L的LiCO₃和/或3.0 µmol/L的Na₂SeO₃·5H₂O处理。随后持续孵育72小时。测定细胞中的总抗氧化状态（TAS）值、抗氧化酶——超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性以及还原型谷胱甘肽浓度（GSH）。
在Vero细胞中，锂降低了所有研究参数，尤其是GPx。硒联合处理显示出明显的保护作用。在FaDu细胞中，仅在GSH方面观察到类似效果。结果表明锂和硒在生理和病理状态下的作用存在差异。由于锂长期用于精神科患者治疗，Vero细胞系的研究结果提示硒可能被视为减轻锂治疗副作用的辅助药物。
