Kiełczykowska Małgorzata, Kocot Joanna, Kurzepa Jacek, Lewandowska Anna, Żelazowska Renata, Musik Irena
Chair and Department of Medical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093, Lublin, Poland.
Biol Trace Elem Res. 2014 Jun;158(3):359-64. doi: 10.1007/s12011-014-9952-4. Epub 2014 Mar 28.
Lithium is widely used in medicine, but its administration can cause numerous side effects. The present study aimed at the evaluation of the possible application of selenium, an essential and antioxidant element, as a protective agent against lithium toxicity. The experiment was performed on four groups of Wistar rats: I (control)-treated with saline, II (Li)-treated with lithium (Li2CO3), III (Se)-treated with selenium (Na2SeO3) and IV (Li + Se)-treated with lithium and selenium (Li2CO3 and Na2SeO3) in the form of water solutions by stomach tube for 6 weeks. The following biochemical parameters were measured: concentrations of sodium, potassium, calcium, magnesium, phosphorus, iron, urea, creatinine, cholesterol, glucose, total protein and albumin and activities of alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase in serum as well as whole blood superoxide dismutase and glutathione peroxidase. Morphological parameters such as red blood cells, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, platelets, white blood cells, neutrophils as well as lymphocytes were determined. Lithium significantly increased serum calcium and glucose (2.65 ± 0.17 vs. 2.43 ± 0.11; 162 ± 31 vs. 121 ± 14, respectively), whereas magnesium and albumin were decreased (1.05 ± 0.08 vs. 1.21 ± 0.15; 3.85. ± 0.12 vs. 4.02 ± 0.08, respectively). Selenium given with lithium restored these parameters to values similar to those observed in the control (Ca-2.49 ± 0.08, glucose-113 ± 26, Mg-1.28 ± 0.09, albumin-4.07 ± 0.11). Se alone or co-administered with Li significantly increased aspartate aminotransferase and glutathione peroxidase. The obtained outcomes let us suggest that the continuation of research on the application of selenium as an adjuvant in lithium therapy seems warranted.
锂在医学中广泛应用，但其给药可引发多种副作用。本研究旨在评估必需的抗氧化元素硒作为锂毒性防护剂的可能应用。实验在四组Wistar大鼠上进行：I组（对照组）用生理盐水处理，II组（锂组）用锂（碳酸锂）处理，III组（硒组）用硒（亚硒酸钠）处理，IV组（锂+硒组）用锂和硒（碳酸锂和亚硒酸钠）以水溶液形式通过胃管给药6周。测量了以下生化参数：血清中钠、钾、钙、镁、磷、铁、尿素、肌酐、胆固醇、葡萄糖、总蛋白和白蛋白的浓度，以及碱性磷酸酶、天冬氨酸转氨酶和丙氨酸转氨酶的活性，还有全血超氧化物歧化酶和谷胱甘肽过氧化物酶。测定了形态学参数，如红细胞、血红蛋白、血细胞比容、平均红细胞体积、平均红细胞血红蛋白、平均红细胞血红蛋白浓度、血小板、白细胞、中性粒细胞以及淋巴细胞。锂显著升高了血清钙和葡萄糖（分别为2.65±0.17对2.43±0.11；162±31对121±14），而镁和白蛋白降低（分别为1.05±0.08对1.21±0.15；3.85±0.12对4.02±0.08）。与锂一起给予的硒将这些参数恢复到与对照组相似的值（钙-2.49±0.08，葡萄糖-113±26，镁-1.28±0.09，白蛋白-4.07±0.11）。单独给予硒或与锂共同给予硒显著增加了天冬氨酸转氨酶和谷胱甘肽过氧化物酶。所获得的结果让我们认为，继续研究将硒作为锂治疗的辅助剂似乎是有必要的。
