Jarzyński Adrian, Zając Przemysław, Żebrowski Remigiusz, Boguszewska Anastazja, Polz-Dacewicz Małgorzata
Department of Virology, Medical University, Lublin, Poland.
Ann Agric Environ Med. 2017 Sep 21;24(3):440-445. doi: 10.26444/aaem/74648. Epub 2017 Jun 9.
Colorectal cancer is one of the most common cancers worldwide. In Poland, it is the second most common cancer, regardless of gender. The aim of study was to analyze the incidence of HPV and BKV in the tissue of colorectal cancer and to determine the relationship between the presence of these viruses and the development of this cancer.
The experiments were conducted using 50 colorectal cancer tissues collected from histological sections. The clinical material was embedded in paraffin blocks. Next, DNA extraction was performed. Isolates of colorectal cancer tissue were tested for the presence of HPV DNA. BKV DNA was detected by PCR using specific primers and then differentiated from JCV by digestion with BamHI enzyme.
In clinical specimens taken from patients with colorectal cancer, HPV DNA was detected in 20% of cases. In 10% of cases the presence of HPV type 18 was confirmed, in the other 90% of the samples HPV type 16 was detected, while the presence of BKV was confirmed in 30% of cases. Coinfection with HPV and BKV was shown in 12% of patients. In one case, BK virus coexisted with HPV type 18, in the remaining 5 cases with HPV type 16.
Developing colorectal cancer can show no symptoms, even for many years. This is why it is so important to become familiar with as many etiological factors as possible. The development of many human neoplasms is often initiated by exposure to infectious agents - such as bacterial or viral infections. Similar to the human papillomavirus, the BK virus was detected in clinical specimens. It seems that HPV and BKV infections can contribute to the neoplastic process, which requires detailed studies on a larger group of patients.
结直肠癌是全球最常见的癌症之一。在波兰，无论性别，它都是第二常见的癌症。本研究的目的是分析人乳头瘤病毒（HPV）和多瘤病毒（BKV）在结直肠癌组织中的发生率，并确定这些病毒的存在与该癌症发展之间的关系。
实验使用从组织切片中收集的50份结直肠癌组织进行。临床材料被包埋在石蜡块中。接下来进行DNA提取。对结直肠癌组织分离物检测HPV DNA的存在情况。使用特异性引物通过聚合酶链反应（PCR）检测BKV DNA，然后用BamHI酶消化与多瘤病毒（JCV）进行区分。
在取自结直肠癌患者的临床标本中，20%的病例检测到HPV DNA。10%的病例确认存在18型HPV，在其他90%的样本中检测到16型HPV，而30%的病例确认存在BKV。12%的患者显示HPV和BKV合并感染。1例中，BK病毒与18型HPV共存，其余5例与16型HPV共存。
结直肠癌在发病时可能没有症状，甚至多年如此。这就是为什么熟悉尽可能多的病因因素非常重要。许多人类肿瘤的发生往往是由接触感染因子引发的，如细菌或病毒感染。与人类乳头瘤病毒类似，在临床标本中检测到了BK病毒。似乎HPV和BKV感染可能促成肿瘤形成过程，这需要对更大规模患者群体进行详细研究。
