胃癌患者中爱泼斯坦-巴尔病毒、巨细胞病毒、人类疱疹病毒6型和多瘤病毒（JC病毒、BK病毒）的调查：一项横断面研究。

Investigation of Epstein-Barr virus, Cytomegalovirus, Human herpesvirus 6, and Polyoma viruses (JC virus, BK virus) among Gastric cancer patients: A cross sectional study.

作者信息

Soltani Saber, Farahani Abbas, Shahbahrami Ramin, Shateri Zainab, Emadi Mohammad Saeid, Pakzad Reza, Lotfi Maryam, Asanjarani Behzad, Rasti Arezoo, Erfani Yousef, Siri Goli

机构信息

Students' Scientific Research Center Tehran University of Medical Sciences Tehran Iran.

Department of Virology School of Public Health, Tehran University of Medical Sciences Tehran Iran.

出版信息

Health Sci Rep. 2024 Apr 21;7(4):e2043. doi: 10.1002/hsr2.2043. eCollection 2024 Apr.

DOI:10.1002/hsr2.2043

PMID:38650724

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11033485/

Abstract

BACKGROUND AND AIMS

Gastric cancer is a significant global issue with a high death rate. This malignancy could be associated with several viral agents such as EBV, CMV, HHV-6, JCV, and BKV.

OBJECTIVE

Evaluation of EBV, CMV, HHV-6 ,and JCV, BKV frequency among gastric cancer patients.

METHODS

In this cross-sectional study, a total number of 60 gastric cancer specimens (32 male, 28 female) were retrieved from the pathology lab. Formalin-fixed paraffin-embedded tissue was used for molecular testing. DNA was extracted from samples, according to protocol, and used for PCR reaction. Polymerase chain reactions were used to assess CMV, EBV, HHV-6, JCV, and BKV frequency.

RESULTS AND CONCLUSION

The mean age of the participants was 61 years and 53.3% (32) of the participants were Male. A total number of 5 samples (8.34%) were infected with viral agents. Four male gastric samples were infected with EBV (6.67%) and only one female sample contained the BKV genome (1.67%). Totally 8.34% of the samples were infected with EBV and BKV. The CMV, HHV-6, and JCV genome was not detected in the samples. In conclusion, the presence of two viral agents including EBV and BKV among male and female samples respectively, and the genome of other viruses were not detected.

摘要

背景与目的

胃癌是一个全球性的重大问题，死亡率很高。这种恶性肿瘤可能与几种病毒病原体有关，如EB病毒（EBV）、巨细胞病毒（CMV）、人疱疹病毒6型（HHV - 6）、多瘤病毒（JCV）和BK病毒（BKV）。

目的

评估胃癌患者中EBV、CMV、HHV - 6、JCV和BKV的感染频率。

方法

在这项横断面研究中，从病理实验室获取了总共60份胃癌标本（男性32例，女性28例）。采用福尔马林固定石蜡包埋组织进行分子检测。按照规程从样本中提取DNA，并用于聚合酶链反应（PCR）。使用聚合酶链反应评估CMV、EBV、HHV - 6、JCV和BKV的感染频率。

结果与结论

参与者的平均年龄为61岁，53.3%（32例）为男性。共有5个样本（8.34%）感染了病毒病原体。4份男性胃癌样本感染了EBV（6.67%），只有1份女性样本含有BKV基因组（1.67%）。总共有8.34%的样本感染了EBV和BKV。样本中未检测到CMV、HHV - 6和JCV基因组。总之，在男性和女性样本中分别存在两种病毒病原体，即EBV和BKV，未检测到其他病毒的基因组。