Tanaka Aya, Era Mariko, Obata Yumeho, Masuda Manami, Kawahara Takayoshi, Kanyama Takahide, Morita Hiroshi
Graduate School of Environmental Engineering, the University of Kitakyushu.
Shabondama Soap Co., Ltd.
Biocontrol Sci. 2017;22(3):153-161. doi: 10.4265/bio.22.153.
Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH. C8K, C10K, and C12K showed growth reduction of 4 log-units (99.99% suppression) in A. castellanii upon 180 min incubation at 175 mM, whereas the pH-adjusted control solution showed no effect. After the amoeba suspension was mixed with C10K or C12K, cell membrane destruction was observed. The minimum inhibitory concentration of C10K and C12K was also determined to be 2.7 mM. Confirmation tests were conducted using contact lenses to evaluate the effectiveness of C10K and C12K as multi-purpose solutions. Experiments using increasing concentrations showed reduced numbers of living cells in C10K (5.5 mM, 10.9 mM) and in C12K (5.5 mM, 10.9 mM). These results demonstrate the inhibitory activity of C10K and C12K against A. castellanii and indicate their potential as anti-amoeba agents.
棘阿米巴存在于海水、淡水和土壤中，是一种机会性病原体，可引起一种可能导致失明的角膜感染，称为棘阿米巴角膜炎。测试了9种脂肪酸盐（丁酸钾（C4K）、己酸盐（C6K）、辛酸盐（C8K）、癸酸盐（C10K）、月桂酸盐（C12K）、肉豆蔻酸盐（C14K）、油酸盐（C18:1K）、亚油酸盐（C18:2K）和亚麻酸盐（C18:3K））对卡氏棘阿米巴ATCC 30010（滋养体和包囊）的抗阿米巴活性。脂肪酸盐（350 mM，pH 10.5）通过将脂肪酸与适量的KOH混合制备。在175 mM下孵育180分钟后，C8K、C10K和C12K使卡氏棘阿米巴的生长减少4个对数单位（99.99%抑制），而pH调节的对照溶液没有效果。将阿米巴悬浮液与C10K或C12K混合后，观察到细胞膜破坏。C10K和C12K的最低抑菌浓度也确定为2.7 mM。使用隐形眼镜进行了确认试验，以评估C10K和C12K作为多功能溶液的有效性。使用浓度递增的实验表明，C10K（5.5 mM，10.9 mM）和C12K（5.5 mM，10.9 mM）中的活细胞数量减少。这些结果证明了C10K和C12K对卡氏棘阿米巴的抑制活性，并表明它们作为抗阿米巴药物的潜力。
