通过[具体名称]的深层培养生产杯伞烷型次生代谢产物及其直接生物自显影法抗菌活性评估。 （注：原文中“and evaluation of their antibacterial activity by direct bioautography.”前缺少具体培养对象，这里补充了“[具体名称]”使句子完整，但根据实际情况可能需要更准确的信息）
Production of cyathane type secondary metabolites by submerged cultures of and evaluation of their antibacterial activity by direct bioautography.
作者信息
Shen T, Morlock G, Zorn H
机构信息
Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany.
Institute of Nutritional Science, and Interdisciplinary Research Center for Biosystems, Land Use and Nutrition, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
出版信息
Fungal Biol Biotechnol. 2015 Dec 22;2:8. doi: 10.1186/s40694-015-0018-y. eCollection 2015.
BACKGROUND
Fungi of the phylum Basidiomycota are well-known to form a broad spectrum of biologically active secondary metabolites, especially low molecular weight compounds such as terpenoids. produces various cyathane type diterpenoids including erinacines. However, no quantitative data and production kinetics have been reported on the biosynthesis of the erinacines C and P in submerged cultures. In the present study, the production of erinacine C was optimized, and the product formation kinetics as well as the antimicrobial activity were studied by high-performance liquid chromatography (HPLC), high-performance thin-layer chromatography (HPTLC) and direct bioautography.
RESULTS
Oatmeal and Edamin K were identified to be crucial media components for an efficient production of erinacine C. The highest concentrations of erinacine C were obtained in the optimized culture medium on the 9 culture day (approximately 260 mg L). The production of erinacine P was strongly time dependent. The maximum concentration of erinacine P of 184 mg L was observed on the third culture day. Afterwards, the concentrations of erinacine P decreased while the concentrations of erinacine C steadily increased. Comparable results were obtained by HPTLC with UV detection and HPLC with diode-array detection (DAD) analyses. Direct bioautography allowed for an additional analysis of the antimicrobial activity of the secondary metabolites.
CONCLUSIONS
The C and N sources oatmeal and Edamin K induced the formation of erinacine C. Detailed product formation kinetics of the erinacines C and P have been reported for the first time. HPTLC combined with the bioassay allowed for an instant detection of cyathane diterpenoids in crude extracts and for an evaluation of the antimicrobial activity of the secondary metabolites directly on the plate.
背景
担子菌门真菌以产生多种具有生物活性的次生代谢产物而闻名，尤其是萜类等低分子量化合物。 能产生包括齿孔酸在内的多种杯伞烷型二萜类化合物。然而，关于在深层培养中齿孔酸C和P的生物合成，尚未有定量数据和生产动力学的报道。在本研究中，对齿孔酸C的生产进行了优化，并通过高效液相色谱（HPLC）、高效薄层色谱（HPTLC）和直接生物自显影技术研究了产物形成动力学以及抗菌活性。
结果
燕麦片和Edamin K被确定为高效生产齿孔酸C的关键培养基成分。在优化培养基中培养9天时获得了最高浓度的齿孔酸C（约260 mg/L）。齿孔酸P的产生强烈依赖于时间。在培养第三天观察到齿孔酸P的最大浓度为184 mg/L。之后，齿孔酸P的浓度下降，而齿孔酸C的浓度稳步上升。通过紫外检测的HPTLC和二极管阵列检测（DAD）分析的HPLC获得了可比结果。直接生物自显影允许对次生代谢产物的抗菌活性进行额外分析。
结论
碳源和氮源燕麦片和Edamin K诱导了齿孔酸C的形成。首次报道了齿孔酸C和P详细的产物形成动力学。HPTLC与生物测定相结合，能够即时检测粗提物中的杯伞烷二萜类化合物，并直接在平板上评估次生代谢产物的抗菌活性。
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