Gössi Angelo, Scherer Uta, Schlotterbeck Götz
University of Applied Sciences Northwestern Switzerland, School of Life Sciences, Institute for Chemistry and Bioanalytics, Gründenstrasse 40, CH-4132 Muttenz, Switzerland.
Chimia (Aarau). 2012;66(5):347-9. doi: 10.2533/chimia.2012.347.
Thin-layer chromatography (TLC) is a mature and very established technique, frequently used in many fields of applications ranging from natural product analysis to chemical or pharmaceutical applications. The introduction of a commercially available TLC-MS interface was a major step complementing the ease of use of TLC with structural elucidation power of mass spectrometry (MS). The TLC-MS interface simplifies the workflow dramatically to gain structural information directly from TLC separations. This article describes the potential of TLC-nuclear magnetic resonance spectroscopy (NMR) utilizing the TLC-MS interface to straightforwardly characterize zones of interest by NMR spectroscopy with a focus on quantification of active pharmaceutical ingredients (API) in formulations and identification of active principles in plant extracts.
薄层色谱法（TLC）是一项成熟且应用广泛的技术，常用于从天然产物分析到化学或制药应用等众多领域。商用TLC-MS接口的引入是一个重要进展，它将TLC的易用性与质谱（MS）的结构解析能力相结合。TLC-MS接口极大地简化了工作流程，可直接从TLC分离中获取结构信息。本文介绍了利用TLC-MS接口进行TLC-核磁共振波谱法（NMR）的潜力，通过NMR光谱直接表征感兴趣的区域，重点是制剂中活性药物成分（API）的定量分析以及植物提取物中活性成分的鉴定。
