不同冲洗液对人根管内金黄色葡萄球菌的杀菌作用
Bactericidal effects of various irrigation solutions against staphylococcus aureus in human root canal.
作者信息
Zan Recai, Kutlu Gizem, Hubbezoglu Ihsan, Sumer Zeynep, Tunc Tutku, Mutlu Zuhal
机构信息
Department of Endodontics, Faculty of Dentistry, Cumhuriyet University Sivas, Turkey.
Department of Restorative, Faculty of Dentistry, Cumhuriyet University Sivas, Turkey.
出版信息
J Istanb Univ Fac Dent. 2015 Jan 31;49(1):19-26. doi: 10.17096/jiufd.70199. eCollection 2015.
PURPOSE
The aim of the present study was to evaluate and to compare the antibacterial effects of various irrigation solutions against Staphylococcus aureus (S. aureus) in human root canals.
MATERIALS AND METHODS
120 single-root mandibular premolar teeth were selected. The teeth were prepared and sterilized. S. aureus was incubated in the root canals and kept at 37°C for 24h. The infected root canals were divided into one positive (saline) and one negative (sodium hypochlorite) control, and four experimental groups [Ethylene-diaminetetra-aceticacid, Chlorhexidine Gluconate, Super-oxidized water(SPO), Aqueous ozone] (n=20). Flow rate of irrigation was applied with 5 mL/min flow rate for 3 min to ensure standardization among all study groups. Following the irrigation, paper points were placed in the root canals and then transferred in sterile eppendorf. Remaining bacteria were counted on blood agar plates and the data were analyzed using one-way ANOVA and Tukey's test.
RESULTS
Although there were statistically significant differences among SPO and other experimental groups (p<0.05), there was no statistically significant difference between SPO and NaOCl (p>0.05).
CONCLUSION
Super-oxidized water may be recommended as an alternative irrigation solution instead of NaOCl against S. aureus in root canals.
目的
本研究旨在评估和比较不同冲洗液对人根管内金黄色葡萄球菌的抗菌效果。
材料与方法
选取120颗单根下颌前磨牙。对牙齿进行预备和消毒。将金黄色葡萄球菌接种于根管内，并在37°C下保存24小时。将感染的根管分为一个阳性对照组（生理盐水）和一个阴性对照组（次氯酸钠），以及四个实验组[乙二胺四乙酸、葡萄糖酸氯己定、超氧化水（SPO）、臭氧水]（n = 20）。以5 mL/min的流速冲洗3分钟，以确保所有研究组之间的标准化。冲洗后，将纸尖放入根管内，然后转移至无菌的微量离心管中。在血琼脂平板上对剩余细菌进行计数，并使用单因素方差分析和Tukey检验对数据进行分析。
结果
虽然SPO与其他实验组之间存在统计学显著差异（p < 0.05），但SPO与NaOCl之间无统计学显著差异（p > 0.05）。
结论
在根管治疗中，超氧化水可作为替代次氯酸钠的冲洗液用于对抗金黄色葡萄球菌。
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