一种基于金纳米颗粒修饰金电极的高灵敏度电化学生物传感器，用于选择性测定血清交联羧基末端肽水平。
A highly sensitive electrochemical biosensor based on AuNP-modified gold electrodes for selective determination of serum levels of crosslaps.
作者信息
Khashayar Patricia, Amoabediny Ghassem, Larijani Bagher, Hosseini Morteza, Verplancke Rik, De Keersmaecker Michel, Adriaens Annemie, Goemaere Stefan, Fiers Tom, Vanfleteren Jan
机构信息
Nanobiotechnology Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
Center for Microsystems Technology, IMEC and Ghent University, Zwijnaarde, Ghent, Belgium.
出版信息
3 Biotech. 2017 Oct;7(5):312. doi: 10.1007/s13205-017-0919-y. Epub 2017 Sep 13.
This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation) and can thus be used for point of care screening. Due to the excellent electrical properties of the as-prepared immunosensor, CTX levels were successfully measured from 1 to 1000 pg/mL. This is while the normal reference of the marker is 50-450 pg/mL, suggesting that the sensor can acceptably detect CTX. The results also showed a good correlation with ECLIA in measuring serum levels of CTX.
本文介绍了一种逐步方案，用于开发一种电化学传感器，以便在几分钟内以高精度定量检测血清中C-末端肽（CTX）交联物（也称为交联环）的水平。该技术仅需一步（孵育），因此可用于即时检测筛查。由于所制备的免疫传感器具有优异的电学性能，成功测量了1至1000 pg/mL的CTX水平。而该标志物的正常参考值为50 - 450 pg/mL，这表明该传感器能够令人满意地检测CTX。结果还显示，在测量血清CTX水平方面，该传感器与电化学发光免疫分析（ECLIA）具有良好的相关性。
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