Mishra Manjita, Singh Shailendra Kumar, Bhardwaj Abhishek, Kumar Lokendra, Singh Manoj Kumar, Sundaram Shanthy
Centre of Biotechnology, University of Allahabad, Prayagraj 211002, Uttar Pradesh, India.
Department of Environmental Science, Veer Bahadur Singh Purvanchal University, Jaunpur 222001, India.
ACS Omega. 2020 Apr 2;5(14):8251-8257. doi: 10.1021/acsomega.0c00551. eCollection 2020 Apr 14.
In India, the major crop is wheat. Its production is severely hampered by seed-borne diseases such as smut and bunt which are responsible for the reduction of crop yield with poor grain quality. In the current study, an attempt was made to prepare a photoluminescence (PL)-based immunosensor for early detection of Karnal bunt (KB) disease. The KB disease-causing pathogen was detected using functionalized diatom frustules as a sensing platform. The teliospore-covered platform, on exposure to light, showed enhanced intensity of PL in comparison to control. This response was directly proportional to the concentration of spores. For the development of a stable frustule-based immunosensor platform, gluteraldehyde was added for the covalent immobilization of the antibody onto amine-functionalized diatom substrates. Frustules of diatom consisting of a nanoporous three-dimensional biogenic silica material exhibit a unique property of emitting strong, visible blue PL under ultraviolet (UV) excitation. PL studies were done to reveal the specificity and binding of the conjugated diatom platform that will distinguish between the (complementary) and (noncomplementary) antigens. Four times better intensity of PL was observed against the complementary one in comparison to a noncomplementary setup (control). The immunocomplex frustule-based platform serves as a suitable sensor platform for early detection of KB.
在印度，主要作物是小麦。其产量受到诸如黑穗病和腥黑穗病等种传疾病的严重阻碍，这些疾病会导致作物产量下降且谷粒品质不佳。在当前研究中，尝试制备一种基于光致发光（PL）的免疫传感器，用于早期检测印度腥黑穗病（KB）。使用功能化的硅藻壳作为传感平台来检测引起KB病的病原体。与对照相比，覆盖冬孢子的平台在光照下显示出增强的PL强度。这种响应与孢子浓度成正比。为了开发稳定的基于硅藻壳的免疫传感器平台，添加戊二醛用于将抗体共价固定在胺功能化的硅藻基质上。由纳米多孔三维生物源二氧化硅材料组成的硅藻壳在紫外（UV）激发下表现出发出强烈可见蓝色PL的独特性质。进行PL研究以揭示共轭硅藻平台的特异性和结合情况，该平台将区分（互补）和（非互补）抗原。与非互补设置（对照）相比，观察到针对互补抗原的PL强度提高了四倍。基于免疫复合物硅藻壳的平台可作为早期检测KB的合适传感器平台。
