Mishra Manjita, Singh Shailendra Kumar, Shanker Rama, Sundaram Shanthy
1Centre of Biotechnology, University of Allahabad, Uttar Pradesh, Prayagraj, 211002 India.
2Department of Civil Engineering, Motilal Nehru National Institute of Technology, Uttar Pradesh, Prayagraj, 211004 India.
3 Biotech. 2020 May;10(5):201. doi: 10.1007/s13205-020-02191-8. Epub 2020 Apr 11.
Fungal pathogen, the cause of Karnal bunt disease in wheat, is severely affecting the yield and grain quality, worldwide. Thus, strict quarantine regulations by most wheat growing countries have to be followed, leading to trade barriers for wheat export. The conventional methods being used for pathogen detection at symptomatic stage requires the germination of spores for the processing of samples. Thus, it is time-consuming and expensive. This study proposes a simulated microcantilever-based piezoelectric biosensor for the early detection of . Four different materials, SiO, SiC, SiN, and Poly Si, were used for the microcantilever design. Microcantilever was coated with siliceous frustules of diatom that provides high surface area and enhanced sensitivity for specific antibody against the antigen, . Ansys software was used for the simulation analysis. Simulation results showed that microcantilever beam of SiO length of 150 µm, width of 30 µm and thickness 1 µm enhanced the sensitivity by two times against the antibody in comparison to normal microcantilever beam. The results concluded that SiO with coated diatom is the best material for the microcantilever fabrication, thus, providing an excellent protocol for fabrication of microcantilever-based biosensor which is both cost- and time effective.
真菌病原体是小麦卡纳尔腥黑穗病的病因，在全球范围内严重影响小麦产量和谷物品质。因此，大多数小麦种植国家必须遵守严格的检疫规定，这导致了小麦出口的贸易壁垒。在症状期用于病原体检测的传统方法需要孢子萌发来处理样本。因此，这种方法既耗时又昂贵。本研究提出了一种基于模拟微悬臂梁的压电生物传感器用于早期检测[病原体名称未给出]。四种不同的材料，SiO、SiC、SiN和多晶硅，被用于微悬臂梁设计。微悬臂梁涂有硅藻的硅质壳，其具有高表面积并增强了对抗抗原[抗原名称未给出]的特异性抗体的灵敏度。使用Ansys软件进行模拟分析。模拟结果表明，长度为150 µm、宽度为3 µm且厚度为1 µm的SiO微悬臂梁与普通微悬臂梁相比，对抗体的灵敏度提高了两倍。结果表明，涂有硅藻的SiO是微悬臂梁制造的最佳材料，因此，为制造基于微悬臂梁的生物传感器提供了一种既经济又省时的优秀方案。
