Mente Eleni, Pierce Graham J, Antonopoulou Efthimia, Stead David, Martin Samuel A M
Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Volos, Greece.
Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, UK.
Biochem Biophys Rep. 2016 Nov 4;9:79-85. doi: 10.1016/j.bbrep.2016.10.012. eCollection 2017 Mar.
Following a meal, a series of physiological changes occurs in animals as they digest, absorb and assimilate ingested nutrients, the kinetics of these responses depends on metabolic rate and nutrient quality. Here we investigated the hepatic proteome in the ectothermic teleost, the rainbow trout, following a single meal to define the postprandial expression of hepatic proteins. The fish were fed a high marine fishmeal/fish oil single meal following a period of 24 h without feeding. Liver protein profiles were examined by 2D gel electrophoresis just before feeding (time 0 h) and at 6 and 12 h after feeding. Of a total of 588 protein spots analysed in a temporal fashion, 49 differed significantly in abundance between the three time groups (ANOVA, p<0.05), before and after feeding, 15 were increased and 34 were decreased in abundance after feeding. Amino acid metabolism-regulated proteins such as phenylalanine-4-hydroxylase and proliferating cell antigen were increased in abundance 12 and 6 h following the meal, suggesting by this time that the fish were increasing their protein turnover to utilize efficiently their dietary protein consumption. Overall, these results highlight some specificity of the trout metabolism and identify postprandial response of metabolism-related proteins 6-12 h after feeding a single meal.
进食后，动物在消化、吸收和同化摄入的营养物质时会发生一系列生理变化，这些反应的动力学取决于代谢率和营养质量。在此，我们研究了变温硬骨鱼虹鳟鱼单次进食后的肝脏蛋白质组，以确定肝脏蛋白质的餐后表达情况。在禁食24小时后，给鱼投喂一顿富含海洋鱼粉/鱼油的食物。在投喂前（0小时）以及投喂后6小时和12小时，通过二维凝胶电泳检测肝脏蛋白质谱。以时间顺序分析的总共588个蛋白点中，三个时间组之间有49个蛋白点的丰度存在显著差异（方差分析，p<0.05），进食前后，有15个蛋白点的丰度增加，34个蛋白点的丰度降低。进食后12小时和6小时，苯丙氨酸-4-羟化酶和增殖细胞核抗原等受氨基酸代谢调节的蛋白质丰度增加，这表明此时鱼正在提高其蛋白质周转率，以有效利用其摄入的膳食蛋白质。总体而言，这些结果突出了虹鳟鱼代谢的一些特异性，并确定了单次进食后6-12小时与代谢相关蛋白质的餐后反应。
