Koziorowska A, Romerowicz-Misielak M, Filipek A, Koziorowski M
Faculty of Mathematics and Natural Sciences, University of Rzeszow, Poland
Laboratory of Bioelectromagnetism, Institute of Biotechnology, University of Rzeszow, Poland
J Biol Regul Homeost Agents. 2017 Jul-Sep;31(3):725-730.
The impact of electromagnetic field (EMF) on humans has been described in numerous studies, but many questions are still unanswered. The aim of the experiment described in this study was to evaluate the effect of EMF on the viability of human fibroblast BJ in vitro and the percentage of cells in different phases of the cell cycle (G1/G0, S, G2/M) after 2 hours of exposure to sinusoidal continuous and pulsed EMFs with frequency of 5 Hz, 60 Hz and 120 Hz at a magnetic induction of 2,5 mT. The viability of BJ cells exposed to an EMF was estimated immediately after completion of exposure and after 24 hours. Metabolic activity of cells was assessed by MTT assay and compared to a control culture not exposed to EMFs. Cell cycle analysis was performed by BrdU incorporation. The analysis of the viability demonstrated significant differences in field efficiency, depending on its nature. Exposure of cells to pulse EMFs resulted in a decrease in their viability for each of the analyzed frequencies. Reduced viability was maintained for a further 24 hours after the end of exposure of cells to pulsed EMF. In the case of continuous field, reduced BJ cell viability was observed only at the highest applied frequency - 120Hz, and this effect maintained for the next 24 hours. Although there was no significant effect on cell viability (metabolic activity) of cells immediately after exposure to continuous EMF with a frequency of 5Hz, a significant increase was observed after 24 hours of incubation.
电磁场（EMF）对人类的影响已在众多研究中有所描述，但仍有许多问题未得到解答。本研究中所描述实验的目的是评估在磁感应强度为2.5 mT时，频率为5 Hz、60 Hz和120 Hz的正弦连续和脉冲电磁场暴露2小时后，电磁场对人成纤维细胞BJ活力以及细胞周期不同阶段（G1/G0、S、G2/M）细胞百分比的影响。在暴露结束后以及24小时后，立即评估暴露于电磁场的BJ细胞的活力。通过MTT法评估细胞的代谢活性，并与未暴露于电磁场的对照培养物进行比较。通过掺入BrdU进行细胞周期分析。活力分析表明，根据场的性质，场效率存在显著差异。将细胞暴露于脉冲电磁场中，对于每个分析频率，细胞活力均下降。在细胞暴露于脉冲电磁场结束后，活力降低的情况持续了另外24小时。对于连续场，仅在最高施加频率120 Hz时观察到BJ细胞活力降低，且这种效应在接下来的24小时内持续存在。尽管在暴露于频率为5 Hz的连续电磁场后，细胞活力（代谢活性）立即没有显著影响，但在孵育24小时后观察到显著增加。
