Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Electromagn Biol Med. 2022 Jan 2;41(1):44-51. doi: 10.1080/15368378.2021.1995872. Epub 2021 Nov 7.
The extremely low frequency electromagnetic field (ELF-EMF) is emerging as a novel approach in cancer treatment. This study evaluated the impact of daily exposure to 50 Hz EMF on breast cancer cells . The MDA-MB-231 and MCF-7 cells were exposed to EMF (50 Hz 20 mT, for 3 hours per day for up to four days) and examined for cell vaibility. The effect of daily ELF-EMF exposure on cell cycle progression and cell death was further investigated. The result revealed that the consecutive exposure to 50 Hz EMF at 20 mT remarkably decreased the viability of MDA-MB-231 compared to the non-exposed group, while it had no significant effect on MCF-7 cells. The ELF-EMF exposure induced G1 phase arrest along with the increase in sub-G1 cell population in MDA-MB-231. Moreover, repeated exposure to 50 Hz EMF promoted cell cycle progression in MCF-7 by increasing the percentage of cells in the S phase. The fluorescent staining revealed that daily exposure of ELF-EMF induced apoptotic cell death in MDA-MB-231, but no morphological change was observed in MCF-7 cells. The results showed that repeated daily exposure to 50 Hz EMF exhibited anti-proliferative activity against invasive breast cancer cells by impairing cell cycle progression and inducing cell death.
极低频电磁场（ELF-EMF）作为一种新的癌症治疗方法正在出现。本研究评估了每天暴露于 50 Hz EMF 对乳腺癌细胞的影响。将 MDA-MB-231 和 MCF-7 细胞暴露于 EMF（50 Hz 20 mT，每天 3 小时，最多持续 4 天），并检查细胞活力。进一步研究了每日 ELF-EMF 暴露对细胞周期进程和细胞死亡的影响。结果表明，与未暴露组相比，连续暴露于 50 Hz EMF 20 mT 可显著降低 MDA-MB-231 的活力，而对 MCF-7 细胞则没有显著影响。ELF-EMF 暴露诱导 G1 期阻滞，同时 MDA-MB-231 中的亚 G1 细胞群增加。此外，重复暴露于 50 Hz EMF 通过增加 S 期细胞的百分比促进 MCF-7 中的细胞周期进程。荧光染色显示，ELF-EMF 的每日暴露诱导 MDA-MB-231 中的凋亡细胞死亡，但 MCF-7 细胞中未观察到形态变化。结果表明，重复每日暴露于 50 Hz EMF 通过损害细胞周期进程和诱导细胞死亡对侵袭性乳腺癌细胞表现出抗增殖活性。
