使用双包层光纤的同步、无标记、多光谱荧光寿命成像和光学相干断层扫描。
Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber.
作者信息
Sherlock Benjamin E, Phipps Jennifer E, Bec Julien, Marcu Laura
出版信息
Opt Lett. 2017 Oct 1;42(19):3753-3756. doi: 10.1364/OL.42.003753.
Abstract
We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.
摘要
我们展示了一种新型的基于光纤的成像平台，该平台使用双包层光纤实现同步荧光寿命成像（FLIm）和光学相干断层扫描（OCT）。通过在样品表面扫描光纤尖端，该平台获取共配准图像，显示未标记生物样品中的结构和成分对比度。在本信函中，我们报告了每种模态的特性，并展示了从柠檬片和一段人类冠状动脉获取的共配准FLIm和OCT图像示例。将FLIm和OCT组合图像与共配准的组织学切片进行的密切比较，为该技术提供了定性验证，并突出了其在组织结构和成分微创多模态成像方面的潜力。
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