Nowicki B, Moulds J, Hull R, Hull S
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Infect Immun. 1988 May;56(5):1057-60. doi: 10.1128/iai.56.5.1057-1060.1988.
A receptor moiety and blood group substance recognized by the O75X adhesin was studied. Well-defined erythrocytes representing different blood group systems and bacterial derivatives carrying plasmid pBJN406 encoding the adhesin were used in a direct hemagglutination assay. We showed that Dr blood group antigen, a component of the IFC blood group complex, is the receptor for the O75X fimbrialike adhesin (Dr hemagglutinin) of uropathogenic Escherichia coli. The molecule recognized by the Dr hemagglutinin on Dr blood group substance is a chloramphenicol-like structure. The inhibitory effect of the active compounds indicates that a tyrosine-containing molecule could be a natural receptor for the Dr hemagglutinin. Dr blood group substance was found in tubular basement membrane and Bowman's capsule of the human kidney. Specific attachment of a Dr hemagglutinin-positive bacterial strain to the kidney substructures was inhibited by chloramphenicol.
对O75X黏附素识别的受体部分和血型物质进行了研究。在直接血凝试验中，使用了代表不同血型系统的明确红细胞以及携带编码该黏附素的质粒pBJN406的细菌衍生物。我们发现，Dr血型抗原是国际法医委员会血型复合物的一个组成部分，是致病性大肠杆菌O75X菌毛样黏附素（Dr血凝素）的受体。Dr血凝素在Dr血型物质上识别的分子是一种氯霉素样结构。活性化合物的抑制作用表明，含酪氨酸的分子可能是Dr血凝素的天然受体。在人肾的肾小管基底膜和鲍曼囊中发现了Dr血型物质。氯霉素可抑制Dr血凝素阳性菌株与肾脏亚结构的特异性附着。
