University Estadual Paulista - UNESP, Dental School of Araçatuba, Department of Surgery and Integrated Clinic, Araçatuba, SP, Brazil.
University of São Paulo - USP, Ribeirão Preto School of Dentistry, Department of Oral and Maxillofacial Surgery and Traumatology and Periodontology, Ribeirão Preto, SP, Brazil.
J Craniomaxillofac Surg. 2017 Nov;45(11):1860-1867. doi: 10.1016/j.jcms.2017.08.011. Epub 2017 Aug 19.
The purpose of this study was to investigate the influence of long-term therapy with intravenous zoledronate (ZA) on the healing of extraction sockets in rats. Forty rats, divided into groups C (Control) and Z (Zoledronate), received intravenous injections of either saline solution or ZA for 24 weeks. Their right maxillary incisor was extracted. Euthanasia was performed at 7 or 28 days postoperative. Histomorphometric (Newly Formed Bone Area) and immunohistochemical (RANKL, OPG and TRAP) analyses were performed. Data were statistically analyzed (ANOVA, Tukey's test and Kruskal-Wallis, Dunn's Multiple Comparison test).Groups C and Z showed similar new bone area, RANKL and OPG immunolabeling. The number of TRAP-positive multinucleated cells was significantly higher in Group Z than in Group C at 28 days. A significantly higher proportion of nonattached osteoclasts were seen in Group Z than in Group C at both periods of analysis. Long-term therapy with intravenous ZA stimulated nonattached osteoclast formation in extraction sockets in rats, thus decreasing local bone resorption. However, it did not influence bone formation by osteoblasts.
本研究旨在探讨长期静脉唑来膦酸（ZA）治疗对大鼠拔牙窝愈合的影响。40 只大鼠分为 C 组（对照组）和 Z 组（唑来膦酸组），分别接受生理盐水或 ZA 静脉注射 24 周。它们的右上颌切牙被拔除。术后 7 天和 28 天进行安乐死。进行组织形态计量学（新骨面积）和免疫组织化学（RANKL、OPG 和 TRAP）分析。数据进行统计学分析（方差分析、Tukey 检验和 Kruskal-Wallis、Dunn's 多重比较检验）。C 组和 Z 组的新骨面积、RANKL 和 OPG 免疫标记相似。在第 28 天，Z 组的 TRAP 阳性多核细胞数量明显高于 C 组。在两个分析期，Z 组的游离破骨细胞比例明显高于 C 组。长期静脉注射 ZA 刺激大鼠拔牙窝中游离破骨细胞的形成，从而减少局部骨吸收。然而，它并没有影响成骨细胞的骨形成。
