Kajimoto Natália de Campos, de Paiva Buischi Yvonne, Loomer Peter Michael, Bromage Timothy G, Ervolino Edilson, Fucini Stephen Enrico, Pola Natália Marcumini, Pirovani Beatriz Ommati, Morábito Maria Juliana Sismeiro, de Almeida Juliano Milanezi, Chaves Furlaneto Flávia Aparecida, Nagata Maria José Hitomi
Department of Periodontics, The University of Texas Health Science Center at San Antonio, San Antonio, TX, USA.
Department of Periodontology and Implant Dentistry, New York University College of Dentistry, New York, NY, USA.
J Periodontal Implant Sci. 2021 Dec;51(6):374-385. doi: 10.5051/jpis.2100700035.
The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy.
Ligature-induced periodontitis was performed in 96 rats. The ligature was tied in the cervical area of the mandibular left first molar. The animals were randomly divided into 4 groups: 1) NT, no treatment; 2) SRP, scaling and root planning; 3) SRP/PLA, SRP followed by filling the periodontal pocket with placebo gel (PLA); and 4) SRP/ALN, SRP followed by filling the periodontal pockets with 1% ALN gel. Histomorphometric (percentage of bone in the furcation region [PBF]) and immunohistochemical (receptor activator of nuclear factor-κB ligand, osteoprotegerin, and tartrate-resistant acid phosphatase) analyses were performed. Data were statistically analyzed, with the threshold of statistical significance set at 0.05.
The SRP, SRP/PLA, and SRP/ALN groups presented a higher PBF than the NT group (≤0.01) at 7, 15, and 30 days. The SRP/ALN group presented a higher PBF than the SRP/PLA group in all experimental periods, as well as a higher PBF than the SRP group at 15 and 30 days. No differences were observed in the immunohistochemical analyses (>0.05 for all).
Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis.
本研究旨在评估局部应用1%阿仑膦酸盐（ALN）凝胶作为非侵入性牙周治疗辅助手段的效果。
对96只大鼠进行结扎诱导性牙周炎。结扎线系于下颌左侧第一磨牙的颈部区域。将动物随机分为4组：1）NT组，不治疗；2）SRP组，龈下刮治和根面平整；3）SRP/PLA组，SRP后用安慰剂凝胶（PLA）填充牙周袋；4）SRP/ALN组，SRP后用1% ALN凝胶填充牙周袋。进行组织形态计量学分析（根分叉区骨百分比[PBF]）和免疫组织化学分析（核因子κB受体活化因子配体、骨保护素和抗酒石酸酸性磷酸酶）。数据进行统计学分析，统计学显著性阈值设定为0.05。
在第7、15和30天，SRP组、SRP/PLA组和SRP/ALN组的PBF均高于NT组（≤0.01）。在所有实验阶段，SRP/ALN组的PBF均高于SRP/PLA组，在第15和30天也高于SRP组。免疫组织化学分析未观察到差异（所有P>0.05）。
在实验性牙周炎大鼠模型中，局部应用1% ALN凝胶作为SRP的辅助手段可增强根分叉区的骨再生。
