Quinn T W, White B N
Department of Biology, Queen's University, Kingston, Ontario, Canada.
Mol Biol Evol. 1987 Mar;4(2):126-43. doi: 10.1093/oxfordjournals.molbev.a040430.
A genomic library of partially EcoRI-digested DNA from the lesser snow goose, Anser caerulescens caerulescens, was constructed in the phage vector Charon 4. Phage containing only unique sequences were identified by screening plaques with 32P-labeled genomic DNA. Restriction-fragment-length polymorphisms (RFLPs) were identified by probing DNA from 11-13 male birds from the breeding colony at La Perouse Bay. Of the 17 probes examined, all detected RFLPs with at least one of EcoRi, HindIII, Msp1, and Taq1. Several of them identified highly variable regions with multiple alleles. These RFLPs are valuable DNA markers that can be used for (1) the examination of DNA variation, relatedness, and genetic distance and (2) assessing paternity and maternity. These data suggest that there are higher levels of variation of DNA sequence in birds than had previously been thought to exist.
利用噬菌体载体Charon 4构建了来自小雪雁（Anser caerulescens caerulescens）的经部分EcoRI酶切的DNA基因组文库。通过用32P标记的基因组DNA筛选噬菌斑，鉴定出仅含有独特序列的噬菌体。通过对拉佩鲁斯湾繁殖群体中11 - 13只雄鸟的DNA进行探针杂交，鉴定出限制性片段长度多态性（RFLP）。在所检测的17个探针中，所有探针都至少在EcoRi、HindIII、Msp1和Taq1中的一种酶切中检测到了RFLP。其中一些探针鉴定出了具有多个等位基因的高度可变区域。这些RFLP是有价值的DNA标记，可用于（1）检测DNA变异、亲缘关系和遗传距离，以及（2）确定亲子关系。这些数据表明，鸟类中DNA序列的变异水平比以前认为的要高。
