Quinn T W
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Mol Ecol. 1992 Aug;1(2):105-17. doi: 10.1111/j.1365-294x.1992.tb00162.x.
By using the polymerase chain reaction to amplify and sequence 178 bp of a rapidly evolving region of the mtDNA genome (segment I of the control region) from 81 individuals, approximately 11% of the variation present in the lesser snow goose Chen caerulescens caerulescens L. mitochondrial genome was surveyed. The 26 types of mtDNA detected formed two distinct mitochondrial clades that differ by an average of 6.7% and are distributed across the species range. Restriction analysis of amplified fragments was then used to assign the mtDNA of an additional 29 individuals to either of these clades. Within one major clade, sequence among mtDNAs was concordant with geographic location. Within the other major clade the degree of sequence divergence among haplotypes was lower and no consistent geographic structuring was evident. The two major clades presumably result from vicariant separation of lesser snow geese during the Pleistocene.
通过聚合酶链反应对81个个体线粒体DNA基因组快速进化区域（控制区片段I）的178bp进行扩增和测序，对小雪雁（Chen caerulescens caerulescens L.）线粒体基因组中约11%的变异进行了调查。检测到的26种线粒体DNA类型形成了两个不同的线粒体分支，平均差异为6.7%，分布于整个物种分布范围。然后通过对扩增片段的限制性分析，将另外29个个体的线粒体DNA归入这两个分支中的一个。在一个主要分支内，线粒体DNA之间的序列与地理位置一致。在另一个主要分支内，单倍型之间的序列差异程度较低，没有明显的一致地理结构。这两个主要分支可能是更新世期间小雪雁地理隔离的结果。
