Martell M, Le Gall I, Millasseau P, Dausset J, Cohen D
Centre d'Etude du Polymorphisme Humain (C.E.P.H.), Paris, France.
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2682-5. doi: 10.1073/pnas.85.8.2682.
Comparison of two different HLA-DQ beta gene sequences from two DR4 individuals, probably corresponding to DQw3.2 (DQR4) and DQw3.1 (DQR5) specificities, has shown several nucleotide variations. Eight oligonucleotides (24 bases long), derived from these polymorphic areas, have been synthesized. Each oligonucleotide was hybridized to BamHI-digested DNA samples from eight families with HLA-DR4 individuals. Four polymorphic BamHI fragments were detected. Two of eight oligonucleotides gave a single signal (8.9 kilobases) on DQw3.2-positive haplotypes. We used one of these oligonucleotides in a genomic DNA dot hybridization and detected a hybridization signal only in DQw3.2-positive individuals. A very simple test like this allows the screening of a large population sample within a very short period.
对两名DR4个体的两种不同HLA - DQβ基因序列进行比较，这两种序列可能分别对应DQw3.2（DQR4）和DQw3.1（DQR5）特异性，结果显示了几个核苷酸变异。从这些多态性区域衍生出了8种寡核苷酸（长度为24个碱基）并进行了合成。每种寡核苷酸都与来自8个有HLA - DR4个体的家庭的经BamHI酶切的DNA样本进行杂交。检测到了4个多态性BamHI片段。8种寡核苷酸中的两种在DQw3.2阳性单倍型上产生了单一信号（8.9千碱基）。我们将其中一种寡核苷酸用于基因组DNA点杂交，并且仅在DQw3.2阳性个体中检测到了杂交信号。这样一个非常简单的检测方法能够在很短时间内对大量人群样本进行筛查。
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