通过寡核苷酸探针检测到的与HLA - DQw3.1和DQw3.2相关的外显子多态性。

HLA-DQw3.1 and DQw3.2 associated exon polymorphisms detected by oligonucleotide probes.

作者信息

Markussen G, Rønningen K S, Paulsen G, Gaudernack G, Sollid L M, Thorsby E

机构信息

Institute of Transplantation Immunology, National Hospital, Oslo, Norway.

出版信息

Tissue Antigens. 1988 Apr;31(4):204-10. doi: 10.1111/j.1399-0039.1988.tb02082.x.

DOI:10.1111/j.1399-0039.1988.tb02082.x

PMID:3406934

Abstract

HLA class II polymorphisms have been analyzed on the genomic level by two oligonucleotide probes corresponding to the DNA sequence coding for the amino acids 23-30 in the first domain of the beta chain of DQw3.1 and DQw3.2. Specific hybridization to single endonuclease fragments of DNA from some HLA homozygous cells was detected. Here we report the distribution of these DNA exon sequences among different DQ alleles, and demonstrate that the probes may be used to type for DQw3.1 and DQw3.2 respectively.

摘要

利用与编码DQw3.1和DQw3.2β链第一结构域中第23 - 30位氨基酸的DNA序列相对应的两种寡核苷酸探针，在基因组水平上分析了HLA - II类多态性。检测到与一些HLA纯合细胞DNA的单核酸内切酶片段的特异性杂交。在此，我们报告了这些DNA外显子序列在不同DQ等位基因中的分布，并证明这些探针可分别用于DQw3.1和DQw3.2的分型。