do Monte Dulciana S, Bezerra Tenório Jonh A, Bastos Isla V G A, de S Mendonça Fábio, Neto Joaquim E, da Silva Teresinha G, Ramos Clécio S
Department of Chemistry, Federal Rural University of Pernambuco, Recife-Pe, Brazil.
Antibiotics Department, Federal University of Pernambuco, Recife-Pe, Brazil.
Toxicol Rep. 2016 Jun 3;3:516-522. doi: 10.1016/j.toxrep.2016.06.001. eCollection 2016.
Considering the increase in consumption of and the use of the compound carotene (BC) as supplement, we investigated potential changes in the chemical and biological proprieties of BC after exposure to smoke (CSS). Our results showed that the BC exposed to CSS underwent 98.8% degradation and suffered loss of its antiradical activity. The major degradation products identified were 3-hydroxy-2,4,4-trimethylpentyl)2-methylpropanoate and (2-ethyl-3-hydroxyhexyl)2-methylpropanoate compounds. These are found in higher levels in the exhalations of colorectal cancer patients and are similar to the toxic products associated with lipid peroxidation of polyunsaturated fatty acids. In toxicological assays using micro-crustacean the BC was non-toxic, while the BC degraded by CSS had a toxicity of LC = 397.35 μg/mL. In Wistar rats, females treated with BC degraded by CSS (BCCSS) showed whitish liver spots, alterations in liver weight and in bilirubin and alkaline phosphatase levels, and decrease in the number of leukocytes associated with atypical lymphocytosis. In male rats, there was an increase in the number of leukocytes when compared to the control group. In the histopathological analysis, the cortical region of the kidneys showed the presence of discrete amorphous eosinophilic material (cylinders) in the lumen of the proximate and distal convoluted tubules. In general, the BC in contact with CSS undergoes chemical changes and exhibits toxicity to rats and .
考虑到[具体物质]的消费量增加以及复合胡萝卜素（BC）作为补充剂的使用，我们研究了BC暴露于[具体烟雾]（CSS）后其化学和生物学特性的潜在变化。我们的结果表明，暴露于CSS的BC发生了98.8%的降解，其抗自由基活性丧失。鉴定出的主要降解产物是3-羟基-2,4,4-三甲基戊基）2-甲基丙酸酯和（2-乙基-3-羟基己基）2-甲基丙酸酯化合物。这些物质在结直肠癌患者的呼出物中含量较高，并且与多不饱和脂肪酸脂质过氧化相关的有毒产物相似。在使用微型甲壳动物的毒理学试验中，BC无毒，而经CSS降解的BC毒性为LC = 397.35μg/mL。在Wistar大鼠中，用经CSS降解的BC（BCCSS）处理的雌性大鼠出现肝白斑、肝脏重量以及胆红素和碱性磷酸酶水平的改变，并且与非典型淋巴细胞增多相关的白细胞数量减少。与对照组相比，雄性大鼠的白细胞数量增加。在组织病理学分析中，肾脏皮质区域在近端和远端曲管腔中显示存在离散的无定形嗜酸性物质（圆柱体）。总体而言，与CSS接触的BC会发生化学变化，并对大鼠和[微型甲壳动物]表现出毒性。
