Hassanpour Azam, Yousefian Shirin, Askaripour Majid, Sharififar Fariba, Ezzatabadipour Massood
Department of Anatomical Sciences, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Toxicol Rep. 2017 Mar 14;4:160-164. doi: 10.1016/j.toxrep.2017.03.002. eCollection 2017.
Evaluation of protective effect of fennel on mouse ovary against the destructive effects of cyclophosphamide (CP) was the aim of this study. Adult female NMARI mice were randomly divided into six groups ( = 8): (A) negative control, (B) CP200 mg/kg, (C) fennel 400 mg/kg/day, (E, F, and D) that received fennel 200, 400 and 100 mg/kg/day respectively + CP200 mg/kg. Their ovary weight, volume, and diameter (WVD) were measured. Five micron sections were stained using the H&E method. The serum levels of oestrogen and progesterone were measured using ELISA kit. The results showed that WVD significantly reduced in the CP-treated groups in comparison with the A and C, but WVD increased after treatment of the mice with fennel extract, in comparison with B group. A significant decrease of serum in terms of oestrogen and progesterone levels among CP-treated groups in comparison with the A group was observed. In the CP-treated groups a reduction in the number of different ovarian follicles in comparison with the A and C groups was observed. However, in the treated animals with fennel extract, these parameters significantly increased in comparison with the B group. Finally, it is concluded that fennel can protect ovary from cyclophosphamide side effects.
本研究旨在评估茴香对小鼠卵巢的保护作用，以对抗环磷酰胺（CP）的破坏作用。成年雌性NMARI小鼠随机分为六组（每组n = 8）：（A）阴性对照组，（B）CP 200 mg/kg组，（C）茴香400 mg/kg/天组，（E、F和D）分别接受茴香200、400和100 mg/kg/天 + CP 200 mg/kg组。测量它们的卵巢重量、体积和直径（WVD）。制作5微米切片，采用苏木精-伊红（H&E）染色法染色。使用酶联免疫吸附测定（ELISA）试剂盒测量血清雌激素和孕酮水平。结果显示，与A组和C组相比，CP处理组的WVD显著降低，但与B组相比，用茴香提取物处理小鼠后WVD增加。与A组相比，观察到CP处理组血清雌激素和孕酮水平显著降低。与A组和C组相比，CP处理组不同卵巢卵泡数量减少。然而，与B组相比，用茴香提取物处理的动物中，这些参数显著增加。最后得出结论，茴香可以保护卵巢免受环磷酰胺的副作用。
