Nsamou Prechmy Carole Ngueyong, Momo Aimé Césaire Tetsatsi, Tchatat Yannick Baudouin Petnga, Fozin Georges Romeo Bonsou, Kemka François Xavier, Ngadjui Esther, Watcho Pierre
Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. BOX. 67, Dschang, Cameroon.
Toxicol Rep. 2022 Mar 8;9:422-431. doi: 10.1016/j.toxrep.2022.03.014. eCollection 2022.
Colibri® a commercial formulation of Imidacloprid severely impairs the reproductive function. This study aimed at evaluating the preventive effects of on the reproductive toxicity of colibri® in female rats. Eighty rats (n = 10/group) were orally treated with colibri® (22.5 mg/kg) and co-administered with either aqueous or methanolic extracts of (55 or 110 mg/kg) within four weeks. Control animals received either distilled water (10 ml/kg), clomiphene citrate or vitamin E. Starting from day 18 of treatment till the end, half of animals in each group (n = 5) was used for the fertility test whereas the remaining rats were kept under treatment until sacrifice. Blood, ovaries, uterus and vagina were collected after sacrifice for measurement of sexual hormones, oxidative stress markers and histological assessment. Exposure of female rats to colibri® was followed by a significant reduction (p < 0.05) in the ovarian and uterine weights, LH, FSH, estradiol and progesterone levels as well as ovarian superoxide dismutase, catalase and peroxidase activities. Moreover, alteration of ovaries, uteri and vagina histology, increase in MDA concentration, decrease in fertility and parturition indices and, pup's viability were recorded. Co-administration of colibri® and plant extracts significantly (p < 0.05-0.001) prevented the above-mentioned damages through biochemical parameter regulations. These results suggest that exerts a preventive effect against colibri®-induced female reproductive toxicity.
可立必®（吡虫啉的一种商业制剂）严重损害生殖功能。本研究旨在评估[某种植物]对可立必®所致雌性大鼠生殖毒性的预防作用。80只大鼠（每组10只）在四周内口服给予可立必®（22.5毫克/千克），并同时给予[某种植物]的水提取物或甲醇提取物（55或110毫克/千克）。对照动物给予蒸馏水（10毫升/千克）、枸橼酸氯米芬或维生素E。从治疗第18天开始直至结束，每组一半动物（每组5只）用于生育力测试，其余大鼠持续接受治疗直至处死。处死动物后采集血液、卵巢、子宫和阴道样本，用于测定性激素、氧化应激标志物以及进行组织学评估。雌性大鼠暴露于可立必®后，卵巢和子宫重量、促黄体生成素（LH）、促卵泡生成素（FSH）、雌二醇和孕酮水平以及卵巢超氧化物歧化酶、过氧化氢酶和过氧化物酶活性均显著降低（p<0.05）。此外，还记录到卵巢、子宫和阴道组织学改变、丙二醛（MDA）浓度升高、生育力和分娩指数降低以及幼崽活力下降。可立必®与植物提取物共同给药通过调节生化参数显著（p<0.05 - 0.001）预防了上述损害。这些结果表明，[某种植物]对可立必®诱导的雌性生殖毒性具有预防作用。
