Watcho Pierre, Carole Nsamou Ngueyong Prechmy, Brice Defo Deeh Patrick, Romeo Bonsou Fozin Georges, Ngadjui Esther, Wankeu-Nya Modeste, Kamtchouing Pierre
Research Unit of Animal Physiology and Phytopharmacology (URPAP), Faculty of Science, University of Dschang, Dschang, Cameroon.
Department of Biomedical Sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Dschang, Dschang, Cameroon.
Int J Reprod Biomed. 2022 Sep 6;20(8):651-662. doi: 10.18502/ijrm.v20i8.11754. eCollection 2022 Aug.
Cyclophosphamide (CP) is an anticancer agent, but its chronic administration induces ovarian toxicity.
We evaluated the effects of aqueous extract (AE) and methanol extract (ME) of ( ) on CP-induced ovarian toxicity in rats.
40 female Wistar rats (10 wk, 170-200 gr) were distributed into 8 groups (n = 5/each) as follows: 1) healthy control; 2) CP+distilled water (10 ml/kg/d); 3) CP+3%-tween 80 (10 mL/kg/d); 4) CP+clomiphene citrate (2 mg/kg/d); 5, 6) CP+AE of (55 and 110 mg/kg/d); and 7, 8) CP+ME of (55 and 110 mg/kg/d). After 28 days of treatment, estrus cyclicity, ovarian and uterine weights as well as estradiol levels and ovarian histology were determined.
CP induced ovarian toxicity after 28 days of exposure. More specifically, CP disturbed the estrus cycle, decreased ovary and uterus weights (p = 0.04), and the 17-β estradiol level (p = 0.04), and induced severe ovarian damages. Remarkably, significantly increased (p = 0.03) the ovarian weight (AE and ME at all doses) and uterus weight (ME at 110 mg/kg/d), compared with the CP-treated rats. Moreover, the 17-β estradiol level was significantly elevated (p = 0.02) in rats given clomiphene citrate and (AE 110 mg/kg/d; ME 55 mg/kg/d). Finally, the ovaries of rats given plant extracts had many corpus luteum and normal follicles, and no cystic follicles.
prevented the detrimental effects of CP on ovarian function, which could support its traditional use as a fertility enhancer.
环磷酰胺（CP）是一种抗癌药物，但其长期给药会诱发卵巢毒性。
我们评估了（ ）的水提取物（AE）和甲醇提取物（ME）对CP诱导的大鼠卵巢毒性的影响。
将40只雌性Wistar大鼠（10周龄，体重170 - 200克）分为8组（每组n = 5），如下：1）健康对照组；2）CP + 蒸馏水（10毫升/千克/天）；3）CP + 3%吐温80（10毫升/千克/天）；4）CP + 枸橼酸氯米芬（2毫克/千克/天）；5、6）CP + （ ）的AE（55和110毫克/千克/天）；7、8）CP + （ ）的ME（55和110毫克/千克/天）。治疗28天后，测定发情周期、卵巢和子宫重量以及雌二醇水平和卵巢组织学。
暴露28天后，CP诱发了卵巢毒性。更具体地说，CP扰乱了发情周期，降低了卵巢和子宫重量（p = 0.04）以及17-β雌二醇水平（p = 0.04），并导致严重的卵巢损伤。值得注意的是，与CP处理的大鼠相比，（ ）显著增加了（p = 0.03）卵巢重量（所有剂量的AE和ME）和子宫重量（110毫克/千克/天的ME）。此外，给予枸橼酸氯米芬和（ ）（AE 110毫克/千克/天；ME 55毫克/千克/天）的大鼠中17-β雌二醇水平显著升高（p = 0.02）。最后，给予植物提取物的大鼠卵巢中有许多黄体和正常卵泡，没有囊性卵泡。
（ ）可预防CP对卵巢功能的有害影响，这可能支持其作为生育增强剂的传统用途。
