Song Ju, Huang Jian, Li Zhi-Dong, Long Yue-Hong, Xing Zhao-Bin
College of Life Sciences, North China University of Science and Technology, Tangshan 063000, China.
College of Agricultural and Food Engineering, Baise University, Baise 533000, China.
Zhongguo Zhong Yao Za Zhi. 2017 Feb;42(4):675-679. doi: 10.19540/j.cnki.cjcmm.20170103.024.
The sweet taste and health effect of Lithocarpus polystachyus are mainly related flavonoid. To obtain Lithocarpus transcriptome database and flavonoid biosynthesis-related genes, the RNA-Seq techology (Illumina HiSeq 4000) was used to sequence its transcriptome. Six Gb database was assembled after assembly steps, and 41 043 of L. polystachyus unigenes were obtained. With blasting them with 7 data banks, all unigenes were involved in 51 GO-terms and 237 metabolic pathways. And furthermore 28 genes of the flavonoid biosynthesis-related were found. After using the MicroSatallite, 18 161 SSR were obtained, the single-nucleotide-repeated was the richest at 7 346. These data represent abundant messages about transcripts and provide valuable genome data sources in molecular biology of L. polystachyus.
多穗柯的甜味和健康功效主要与黄酮类化合物有关。为了获得多穗柯转录组数据库和黄酮类生物合成相关基因，采用RNA-Seq技术（Illumina HiSeq 4000）对其转录组进行测序。经过组装步骤后组装出了6Gb的数据库，并获得了多穗柯4,1043条单基因。通过与7个数据库进行比对，所有单基因涉及51个GO术语和237条代谢途径。此外，还发现了28个黄酮类生物合成相关基因。利用微卫星技术后，获得了18,161个SSR，其中单核苷酸重复最为丰富，有7,346个。这些数据代表了关于转录本的丰富信息，并为多穗柯分子生物学提供了有价值的基因组数据源。
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