Wan Kui, Ban Jingjie, Yang Fengjie, Zhang Xueying, Huang Xiaoling, Wang Yanqiu, Zhang Zihao, Lai Zhongxiong, Chen Yukun, Lin Yuling
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2024 May 31;13(11):1524. doi: 10.3390/plants13111524.
Hua () rhizomes are rich in flavonoids and other secondary metabolites, exhibiting remarkable antioxidant, anti-tumor, and immunomodulatory effects. flavonoid-biosynthesis-related genes have been characterized already. However, a comprehensive overview of flavonoid biosynthesis pathways is still absent. To articulate the accumulation of the flavonoid biosynthesis pathways, we examined transcriptome changes using Illumina HiSeq from five different tissues and the RNA-seq of 15 samples had over 105 Gb of a clean base, generating a total of 277,955 unigenes. The cDNA libraries of the fruits (F), leaves (L), roots (R), stems (S), and rhizomes (T) of three-year-old plants generated 57,591, 53,578, 60,321, 51,530, and 54,935 unigenes. Comparative transcriptome analysis revealed that 379 differentially expressed genes (DEGs) were in the group of F vs T, L vs T, R vs T, and S vs T, and the transcripts of flavonoid-biosynthesis-related DEGs were principally enriched in rhizomes. In addition, combined with WGCNA and the FPKM of five tissues' transcription, nine differentially expressed transcription factor families (, , , etc.) were characterized in the red module, the red module positively correlated with rhizome flavonoid accumulation. Quantitative real-time PCR (qRT-PCR) further indicated that , , , and are differentially expressed in rhizomes, accompanied in rhizome development in . Therefore, this study provides a foundation for further research into uncovering the accumulation of flavonoid biosynthesis in the rhizomes of .
华（）根茎富含黄酮类化合物和其他次生代谢产物，具有显著的抗氧化、抗肿瘤和免疫调节作用。与黄酮生物合成相关的基因已被鉴定。然而，黄酮生物合成途径的全面概述仍然缺乏。为了阐明黄酮生物合成途径的积累，我们使用Illumina HiSeq检测了来自五个不同组织的转录组变化，15个样本的RNA-seq有超过105 Gb的 clean base，共产生了277,955个单基因。三年生植物的果实（F）、叶片（L）、根（R）、茎（S）和根茎（T）的cDNA文库分别产生了57,591、53,578、60,321、51,530和54,935个单基因。比较转录组分析显示，在F_vs_T、L_vs_T、R_vs_T和S_vs_T组中有379个差异表达基因（DEG），与黄酮生物合成相关的DEG转录本主要富集在根茎中。此外，结合WGCNA和五个组织转录的FPKM，在红色模块中鉴定出九个差异表达的转录因子家族（、、、等），红色模块与根茎黄酮积累呈正相关。定量实时PCR（qRT-PCR）进一步表明，、、和在根茎中差异表达，伴随着的根茎发育。因此，本研究为进一步研究揭示华根茎中黄酮生物合成的积累提供了基础。
