Zhang Duoduo, Wang Shuqing, Lin Limei, Zhang Jie, Cui Minghui, Wang Shuo, Zhao Xuelei, Dong Jing, Long Yuehong, Xing Zhaobin
College of Life Sciences, North China University of Science and Technology, Tangshan 063210, China.
Hospital of North China University of Science and Technology, Tangshan 063210, China.
ACS Omega. 2022 May 31;7(23):19437-19453. doi: 10.1021/acsomega.2c01125. eCollection 2022 Jun 14.
Rehd has received great attention because of its pharmacological activities, such as inhibiting oxidation and lowering blood glucose and blood pressure, and flavonoids are one of its main pharmacodynamic components. It is important to understand the mechanisms of the flavonoid biosynthetic pathway of , but the regulation of flavonoid biosynthesis is still unclear. In this study, differentially expressed genes and differentially accumulated metabolites in were studied by integrating transcriptomics and metabolomics technologies. We confirmed the key genes involved in the flavonoid biosynthesis of , including , , , , and , which had consistent expression patterns with their upstream and downstream metabolites, and there is a significantly positive correlation between them. Compared to mature leaves, stems and young leaves are higher in the expression levels of key structural genes. We deduced that the MYB and bHLH transcription factors regulated the biosynthesis of different flavonoid metabolites and their regulatory patterns. Among them, , , , , and positively regulated the biosynthesis of flavones and flavanones. This discovery preliminarily revealed the pathways and key genes of flavonoid biosynthesis in , which provided a reference for further study on flavonoid biosynthesis.
由于其药理活性，如抑制氧化、降低血糖和血压，[植物名称未明确，推测为某种植物，暂用Rehd指代]受到了广泛关注，黄酮类化合物是其主要药效成分之一。了解Rehd黄酮类生物合成途径的机制很重要，但黄酮类生物合成的调控仍不清楚。在本研究中，通过整合转录组学和代谢组学技术，研究了Rehd中差异表达基因和差异积累的代谢物。我们确定了参与Rehd黄酮类生物合成的关键基因，包括[基因名称未明确，暂用相关字母指代]，它们与其上游和下游代谢物具有一致的表达模式，并且它们之间存在显著的正相关。与成熟叶相比，茎和幼叶中关键结构基因的表达水平更高。我们推测MYB和bHLH转录因子调控不同黄酮类代谢物的生物合成及其调控模式。其中，[相关基因名称未明确，暂用相关字母指代]正向调控黄酮和黄烷酮的生物合成。这一发现初步揭示了Rehd中黄酮类生物合成的途径和关键基因，为进一步研究黄酮类生物合成提供了参考。
