School of Chemistry, University of Birmingham, Edgbaston, B15 2TT, UK.
Institute of Cardiovascular Sciences, University of Birmingham, Edgbaston, B15 2TT, UK.
Nanomedicine (Lond). 2017 Nov;12(22):2725-2740. doi: 10.2217/nnm-2017-0211. Epub 2017 Sep 29.
Imaging of blood flow in narrow channels and close to vessel walls is important in cardiovascular research for understanding pathogenesis. Our aim was to provide novel nanoprobes with visible emission and long lifetimes as trackers of flow.
MATERIALS & METHODS: Gold nanoparticles coated with an iridium complex were prepared. Luminescence imaging was used to monitor their flows in different hematocrit blood and in murine tissues.
The velocities are independent of hematocrit level and the nanoparticles entering blood circulation can be clearly detected in vessels in lungs, mesentery and the skeletal muscle.
The work introduces for the first time iridium-based yellow-green luminescence with nanoparticle size of 100 nm for visualizing and monitoring flows with much higher resolution than conventional alternatives.
在心血管研究中，对狭窄通道和靠近血管壁的血流成像对于了解发病机制非常重要。我们的目的是提供具有可见发射和长寿命的新型纳米探针，作为流动的示踪剂。
制备了包覆铱配合物的金纳米颗粒。利用发光成像监测它们在不同血细胞比容的血液中和在小鼠组织中的流动。
速度与血细胞比容水平无关，进入血液循环的纳米颗粒可以在肺部、肠系膜和骨骼肌中的血管中清晰检测到。
该工作首次引入了基于铱的黄绿光，纳米颗粒尺寸为 100nm，用于可视化和监测流动，分辨率远高于传统方法。
