Kiss J, Csáky A, Halász B
Second Department of Anatomy, Semmelweis University Medical School, Budapest, Hungary.
Brain Res. 1988 Feb 23;442(1):23-32. doi: 10.1016/0006-8993(88)91428-x.
Using a combination of electron microscopic autoradiography and immunocytochemistry, the connections between serotoninergic axons and somatostatin neurons of the anterior periventricular nucleus of the rat hypothalamus were examined. The serotoninergic elements were identified after selective uptake of tritiated serotonin and the somatostatin neurons with immunocytochemistry. Synaptic connections between labeled serotoninergic nerve endings and somatostatin-immunoreactive neurons were observed. This finding provides morphological evidence for a direct influence of serotoninergic elements on somatostatin neurons of the anterior periventricular nucleus projecting to the median eminence of the hypothalamus.
运用电子显微镜放射自显影术和免疫细胞化学相结合的方法，研究了大鼠下丘脑室周前核中5-羟色胺能轴突与生长抑素神经元之间的联系。通过选择性摄取氚标记的5-羟色胺来鉴定5-羟色胺能成分，并用免疫细胞化学方法鉴定生长抑素神经元。观察到标记的5-羟色胺能神经末梢与生长抑素免疫反应性神经元之间的突触联系。这一发现为5-羟色胺能成分对投射到下丘脑正中隆起的室周前核生长抑素神经元的直接影响提供了形态学证据。
