Microbial Biotechnology Division, Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
Quality Control and Quality Assurance Division, Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India.
BMC Microbiol. 2019 May 16;19(1):98. doi: 10.1186/s12866-019-1477-8.
Exploration of microbes isolated from north western Himalayas for bioactive natural products.
A strain of Trichoderma lixii (IIIM-B4) was isolated from Bacopa monnieri L. The ITS based rDNA gene sequence of strain IIIM-B4 displayed 99% sequence similarity with different Trichoderma harzianum species complex. The highest score was displayed for Hypocrea lixii strain FJ462763 followed by H. nigricans strain NBRC31285, Trichoderma lixii strain CBS 110080, T. afroharzianum strain CBS124620 and Trichoderma guizhouense BPI:GJS 08135 respectively. Position of T. lixii (IIIM-B4) in phylogenetic tree suggested separate identity of the strain. Microbial dynamics of T. lixii (IIIM-B4) was investigated for small peptides. Medium to long chain length peptaibols of 11 residue (Group A), 14 residue (Group B) and 17 residue (Group C) were identified using Matrix Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometer. Optimization is undeniably a desideratum for maximized production of desirable metabolites from microbial strain. Here optimization studies were carried out on T. lixii (IIIM-B4) using different growth media through Intact Cell Mass Spectrometry (ICMS). A multifold increase was obtained in production of 11 residue peptaibols using rose bengal medium. Out of these, one of them named as Tribacopin AV was isolated and sequenced through mass studied. It was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH. It was found to have antifungal activity against Candida albicans (25 μg/mL MIC).
In this study, we isolated a strain of T. lixii (IIIM-B4) producing medium and long chain peptaibols. One of them named as Tribacopin AV was found novel as having unique sequence Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH, which had antifungal properties.
探索来自喜马拉雅山西北部的微生物以获得生物活性天然产物。
从 Bacopa monnieri L 中分离出一株 Trichoderma lixii（IIIM-B4）。菌株 IIIM-B4 的基于 ITS 的 rDNA 基因序列与不同的 Trichoderma harzianum 种复合体显示出 99%的序列相似性。得分最高的是 Hypocrea lixii 菌株 FJ462763，其次是 H. nigricans 菌株 NBRC31285、Trichoderma lixii 菌株 CBS 110080、Trichoderma afroharzianum 菌株 CBS124620 和 Trichoderma guizhouense BPI:GJS 08135。在系统发育树中 Trichoderma lixii（IIIM-B4）的位置表明该菌株具有单独的身份。研究了 Trichoderma lixii（IIIM-B4）的微生物动力学，以寻找小肽。使用基质辅助激光解吸/离子化-飞行时间（MALDI-TOF）质谱仪鉴定了 11 个残基（A 组）、14 个残基（B 组）和 17 个残基（C 组）的中长链肽聚糖。优化是从微生物菌株中最大化生产所需代谢物的不可或缺的要求。在这里，通过完整细胞质谱法（ICMS）在不同的生长培养基上对 Trichoderma lixii（IIIM-B4）进行了优化研究。在使用孟加拉玫瑰培养基时，11 个残基肽聚糖的产量增加了多倍。其中一种名为 Tribacopin AV 的物质被分离出来，并通过质谱研究进行了测序。它被发现是新颖的，因为它具有独特的序列 Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH。它对 Candida albicans（25μg/mL MIC）具有抗真菌活性。
在这项研究中，我们分离出一株 Trichoderma lixii（IIIM-B4）产生中长链肽聚糖的菌株。其中一种名为 Tribacopin AV 的物质被发现是新颖的，因为它具有独特的序列 Ac-Gly-Leu-Leu-Leu-Ala-Leu-Pro-Leu-Aib-Val-Gln-OH，具有抗真菌特性。
